Mahler1: Subphenotypes of colitis susceptibility in IL-10-deficient mice generated from backcrosses involving C3H/HeJBir and C57BL/6J (2002)

Mahler M, Most C, Schmidtke S, Sundberg JP, Li R, Hedrich HJ, Churchill GA. Genetics of colitis susceptibility in IL-10-deficient mice: backcross versus F2 results contrasted by principal component analysis. Genomics. 2002 Sep;80(3):274-82.   PubMed 12213197  


       
Backcrosses involving C3H/HeJBir and C57BL/6J inbred mouse strains homozygous for the disrupted Il10 gene were generated to identify QTLs involved in colitis susceptibility. Spleens were assessed in backcross progeny and the cecum and colon were scored for lesions based on severity, ulceration, hyperplasia, and percentage of area involved. For detailed protocols see Mahler et al 2002.

Note on studied population: C3H/HeJBir x C57BL/6J backcrosses

Procedures conducted:
• study archive  Colitis susceptibility. Colon histopathology, spleen weight.

Mahler1 downloads
QTL projects have several available downloads.
Use the QTL Data Downloads button near the bottom of this page.
Investigators M Mahler
Gary A Churchill       The Jackson Laboratory,  Bar Harbor, ME
Participants Most C, Schmidtke S, Sundberg JP, Li R, Hedrich HJ
ContactGary A Churchill     gary.churchill@jax.org
Affiliated CenterQTL Archive
AcknowledgementsFunding provided by NIH DK44240, CA34196; and the Deutsche Forschungsgemeinschaft
Project type QTL Archive data set
MPD identifiersMahler1     MPD:121
Data changelog No updates/corrections.       Initial release date: 01/2008.
QTL data set parameters:
Cross 1: (C3H x B6) x B6 backcross       Mahler1
Progenitor strains B6.Il10-/-     C3H.Il10-/-    
Cross direction notation(C3H x B6) x B6
Cross typeBackcross
Population size 100 ♀     103 ♂
NotesN2 progeny
Age6wks

Progenitor strains B6.Il10-/-     C3H.Il10-/-    
Cross typeNone
Population size None ♀     None ♂
Age6wks


Measured phenotypes:       To access these data use the QTL Data Downloads button at bottom of page.
Data set
Procedure / Protocol
Treatment
Phenotype Measure / Variable (click to view)
Panel
No. of Strains
Sex
Age
Sample Size (Avg)
Mahler1 organ weights (none) spleen to body weight ratio, first Principal Component   [ratio]  SplBW   12101 QTL population both 6wks N=102
Mahler1 histopathology (none) cecum assessment, first Principal Component  CecumPC1   12102 QTL population both 6wks N=102
Mahler1 histopathology (none) proximal colon assessment, first Principal Component  ProxPC1   12103 QTL population both 6wks N=102
Mahler1 histopathology (none) middle colon assessment, first Principal Component  MidPC1   12104 QTL population both 6wks N=102
Mahler1 histopathology (none) distal colon assessment, first Principal Component  DistPC1   12105 QTL population both 6wks N=102
Mahler1 histopathology (none) ulceration (score 0-4, increasing severity), first Principal Component  Ulceration-PC1   12106 QTL population both 6wks N=102
Mahler1 histopathology (none) ulceration (score 0-4, increasing severity), second Principal Component  Ulceration-PC2   12107 QTL population both 6wks N=102

Associated genomic features
MGI-curated QTL / genomic features that have been associated with this data set:
Cdcs10   Cdcs7   Cdcs8   Cdcs9  


Cross 1: (C3H x B6) x B6 backcross     Mahler1     a.k.a. Mahler_2002a
Excel file:   Data_Description_C3HxB6_Mahler2002a.xlsx
CSV file:   Mahler2002a_B6BC_B37_Data.csv
Readme / text file:   readme_Mahler2002a.txt
QC plots, figures, etc.:   QCReport_Mahler2002a.pdf

Project / data set:   Mahler1b     a.k.a. None

QTL file list not available on this MPD instance.