MPD: Data set: Mahler1

Mahler1: Subphenotypes of colitis susceptibility in IL-10-deficient mice generated from backcrosses involving C3H/HeJBir and C57BL/6J (2002)

Mahler M, Most C, Schmidtke S, Sundberg JP, Li R, Hedrich HJ, Churchill GA. Genetics of colitis susceptibility in IL-10-deficient mice: backcross versus F2 results contrasted by principal component analysis. Genomics. 2002 Sep;80(3):274-82. PubMed 12213197

Mahler1 downloads
QTL projects have several available downloads.
Use the QTL Data Downloads button near the bottom of this page.

Ontology terms mapped to Mahler1 measures:

Investigators	M Mahler Gary A Churchill The Jackson Laboratory, Bar Harbor, ME
Participants	Most C, Schmidtke S, Sundberg JP, Li R, Hedrich HJ
Contact	Gary A Churchill gary.churchill@jax.org
Affiliated Center	QTL Archive
Acknowledgements	Funding provided by NIH DK44240, CA34196; and the Deutsche Forschungsgemeinschaft
Project type	QTL Archive data set
MPD identifiers	Mahler1 MPD:121
Data changelog	No updates/corrections. Initial release date: 01/2008.

Backcrosses involving C3H/HeJBir and C57BL/6J inbred mouse strains homozygous for the disrupted Il10 gene were generated to identify QTLs involved in colitis susceptibility. Spleens were assessed in backcross progeny and the cecum and colon were scored for lesions based on severity, ulceration, hyperplasia, and percentage of area involved. For detailed protocols see Mahler et al 2002.

QTL data set parameters:

Cross 1: (C3H x B6) x B6 backcross Mahler1
Progenitor strains	B6.Il10^-/- C3H.Il10^-/-
Cross direction notation	(C3H x B6) x B6
Cross type	Backcross
Population size	100 ♀ 103 ♂
Notes	N2 progeny
Age	6wks

Cross 2: (C3H x B6) x C3H backcross Mahler1b
Progenitor strains	B6.Il10^-/- C3H.Il10^-/-
Cross direction notation	(C3H x B6) x C3H
Cross type	Backcross
Population size	116 ♀ 143 ♂
Age	6wks

Measured phenotypes: To access these data use the QTL Data Downloads button at bottom of page.

Data set	Procedure / Protocol	Treatment	Phenotype Measure / Variable (click to view)	Panel	Sex	Age	Sample Size (Avg)
Mahler1	organ weights	(none)	spleen to body weight ratio, first Principal Component [ratio] SplBW 12101	QTL population	both	6wks	N=102

Mahler1	histopathology	(none)	cecum assessment, first Principal Component CecumPC1 12102	QTL population	both	6wks	N=102

Mahler1	histopathology	(none)	proximal colon assessment, first Principal Component ProxPC1 12103	QTL population	both	6wks	N=102

Mahler1	histopathology	(none)	middle colon assessment, first Principal Component MidPC1 12104	QTL population	both	6wks	N=102

Mahler1	histopathology	(none)	distal colon assessment, first Principal Component DistPC1 12105	QTL population	both	6wks	N=102

Mahler1	histopathology	(none)	ulceration (score 0-4, increasing severity), first Principal Component Ulceration-PC1 12106	QTL population	both	6wks	N=102

Mahler1	histopathology	(none)	ulceration (score 0-4, increasing severity), second Principal Component Ulceration-PC2 12107	QTL population	both	6wks	N=102

Associated genomic features

MGI-curated QTL / genomic features that have been associated with this data set:
Cdcs10 Cdcs7 Cdcs8 Cdcs9

Cross 1: (C3H x B6) x B6 backcross Mahler1 a.k.a. Mahler_2002a
Excel file: Data_Description_C3HxB6_Mahler2002a.xlsx
CSV file: Mahler2002a_B6BC_B37_Data.csv
Readme / text file: readme_Mahler2002a.txt
QC plots, figures, etc.: QCReport_Mahler2002a.pdf

Cross 2: (C3H x B6) x C3H backcross Mahler1b a.k.a. Mahler_2002b
Excel file: Data_Description_C3HxB6_Mahler2002b.xlsx
CSV file: Mahler2002b_C3HBC_B37_Data.csv
Readme / text file: readme_Mahler2002b.txt
QC plots, figures, etc.: QCReport_Mahler2002b.pdf