Maeda1: Genetic architecture of atherosclerosis dissected by QTL analysis in an intercross of apolipoprotein E-null mice on C57BL/6J and DBA/2J backgrounds (2017)

Makhanova N, Morgan AP, Kayashima Y, Makhanov A, Hiller S, Zhilicheva S, Xu L, Pardo-Manuel de Villena F, Maeda N. Genetic architecture of atherosclerosis dissected by QTL analyses in three F2 intercrosses of apolipoprotein E-null mice on C57BL6/J, DBA/2J and 129S6/SvEvTac backgrounds. PLoS One. 2017 Aug 24;12(8):e0182882. doi: 10.1371/journal.pone.0182882. eCollection 2017.   PubMed 28837567  


     
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Participants Nobuyo Maeda       University of North Carolina Chapel Hill, NC
With: Makhanova N, Morgan AP, Kayashima Y, Makhanov A, Hiller S, Zhilicheva S, Xu L, Pardo-Manuel de Villena F
ContactNobuyo Maeda     nobuyo@med.unc.edu
AffiliationChurchill Group QTL Archive
AcknowledgementsFunding provided by HL042630
Project type QTL Archive data set
MPD identifiersMaeda1     MPD:603
Data changelog No updates/corrections.       Initial release date: 08/2017.
We analyzed F2 progeny from a cross between apolipoprotein E-null mice on DBA/2J (DBA-apoE) and C57BL/6J (B6-apoE) genetic backgrounds Apoe-null mice on DBA and B6 backgrounds were obtained from the Jackson Laboratory (Bar Harbor, ME, USA). Male DBA-apoE mice were mated to female B6-apoE mice to generate F1 progeny. F1 hybrids were intercrossed to generate the F2 population. F2 mice (136 females and 96 males) aged 4.5 months were used for analyses in this study. Sizes of atherosclerotic plaques in the aortic root and the aortic arch were measured as described previously (Tomita et al, 2010; Zhang SH et al 1992). Briefly, mice were euthanized with a lethal dose of 2,2,2-tribromoethanol followed by cervical dislocation, and perfused with 4% paraformaldehyde through the left ventricle of the heart. The plaque areas in the defined locations of the aortic arch (FigS1) were measured using images captured with Image J software. The plaques at the innercurve of the aortic arch were used to represent “arch plaque size”. Lesion sizes (in micrometers squared) at the inner curvature of aortic arch and at the aortic root were subject to square root transformation to achieve an approximately normal data distribution.


QTL data set parameters:
Cross direction notation(B6-ApoE x DBA-ApoE)F1 x (B6-ApoE x DBA-ApoE)F1
Cross typeF2 intercross
Population size 136 ♀     96 ♂
Age18-20wks


Measured phenotypes:       To access these data use the QTL Data Downloads button at bottom of page.
Data set MPD ID Procedure / Protocol Drug or Challenge Phenotype Measure Panel No. of Strains Sex Age Sample Size (Avg)
Maeda1 60301 histopathology (none) aortic lesion size at root, square root transformed   [µm2]  Sqrt_Root QTL population both 18-20wks N=116
Maeda1 60302 histopathology (none) lesion size at aortic arch, square root transformed   [µm2]  Sqr_Area1 QTL population both 18-20wks N=116
Maeda1 60303 histopathology (none) lesion size at innominate artery, square root transformed   [µm2]  Sqr_Area2 QTL population both 18-20wks N=116
Maeda1 60304 histopathology (none) lesion size at left common carotid artery, square root transformed   [µm2]  Sqr_Area3 QTL population both 18-20wks N=116
Maeda1 60305 histopathology (none) lesion size at subclavian artery plus upper wall, square root transformed   [µm2]  Sqr_Area4 QTL population both 18-20wks N=116



Project / data set:   Maeda1     a.k.a. Maeda_2017
CSV file:   Maeda1_cross.csv