Lyons2: Gallstone susceptibility in F2 progeny of 129S1/SvImJ and CAST/EiJ (2003)

References   (chronological)

1.   Lyons MA, Wittenburg H, Li R, Walsh KA, Leonard MR, Churchill GA, Carey MC, Paigen B. New quantitative trait loci that contribute to cholesterol gallstone formation detected in an intercross of CAST/Ei and 129S1/SvImJ inbred mice. Physiol Genomics. 2003 Aug 15;14(3):225-39.   PubMed 12837957  

2.   Lyons MA, Wittenburg H, Li R, Walsh KA, Korstanje R, Churchill GA, Carey MC, Paigen B. Quantitative trait loci that determine lipoprotein cholesterol levels in an intercross of 129S1/SvImJ and CAST/Ei inbred mice. Physiol Genomics. 2004 Mar 12;17(1):60-8.   PubMed 14701919  

3.   Li R, Lyons MA, Wittenburg H, Paigen B, Churchill GA. Combining data from multiple inbred line crosses improves the power and resolution of quantitative trait loci mapping. Genetics. 2005 Mar;169(3):1699-709. Epub 2005 Jan 16.   PubMed 15654110     FullText


       
To determine the genetic contribution to variation among cholesterol levels, an F2 intercross between RIIIS/J and 129S1/SvImJ strains was generated. 330 male mice from F2 population were fed an atherogenic (high-fat) diet for 8-12 wks. The total plasma, HDL and non-HDL were determined at the end of feeding period and some significant QTLs were identified in these phenotypes. For detailed protocols see Lyons et al 2004.

Note on studied population: 129S1/SvImJ x CAST/EiJ F2 progeny

Procedures conducted:
• study archive  Lipids (plasma), bile, liver weight, gallbladder and gallstone histopathology.

Lyons2 downloads
QTL projects have several available downloads.
Use the QTL Data Downloads button near the bottom of this page.
Investigators M A Lyons
Gary A Churchill       The Jackson Laboratory,  Bar Harbor, ME
Participants Wittenburg H, Li R, Walsh KA, Korstanje R, Carey MC, Paigen B
ContactGary A Churchill     gary.churchill@jax.org
Affiliated CenterQTL Archive
AcknowledgementsThis work was supported in part by National Institute Health Grants DK-51568 (to B. Paigen), DK-36588, DK-52911, and DK-34854 (to M. C. Carey), and CA-34196 (core grant to the Jackson Laboratory). M. A. Lyons was supported by the American Physiological Society and the American Liver Foundation.
Project type QTL Archive data set
MPD identifiersLyons2     MPD:131
Data changelog No updates/corrections.       Initial release date: 01/2008.
QTL data set parameters:
Progenitor strains 129S1/SvImJ     CAST/EiJ    
Cross direction notation(CASTx129) F1 x (CASTx129) F1; (129xCAST) F1 x (129xCAST) F1
Cross typeF2 intercross
Population size 0 ♀     277 ♂
Age16-18wks


Measured phenotypes:       To access these data use the QTL Data Downloads button at bottom of page.
Select table page: First Prev 1 - 10 / 14 (14) Next Last
Data set
Procedure / Protocol
Treatment
Phenotype Measure / Variable (click to view)
Panel
No. of Strains
Sex
Age
Sample Size (Avg)
Lyons2 organ weights high-fat diet liver weight   [g]  liver_wt   13101 QTL population m 16-18wks N=277
Lyons2 organ weights high-fat diet liver-to-bw ratio   [ratio]  liver_bw_ratio   13102 QTL population m 16-18wks N=277
Lyons2 organ dimensions high-fat diet gallbladder, volume   [µL]  gallbladder_vol   13103 QTL population m 16-18wks N=277
Lyons2 histopathology high-fat diet gallbladder, mucin (score 0-4)   [score]  gallbladder_mucin   13104 QTL population m 16-18wks N=277
Lyons2 histopathology high-fat diet gallbladder bile characteristics (score 0=no crystals, 1=crystals present, 2=solid or sandy gallstones present)   [score]  bile_score   13105 QTL population m 16-18wks N=277
Lyons2 histopathology high-fat diet cholesterol monohydrate crystals (score 0=absent, 1-4=increasing severity)   [score]  gallstone_crystals   13106 QTL population m 16-18wks N=277
Lyons2 histopathology high-fat diet sandy gallstones (score 0=absence, 1-4=severity)   [score]  gallstone_sandy   13107 QTL population m 16-18wks N=277
Lyons2 histopathology high-fat diet solid gallstones (score 0=absence, 1-5=severity)   [score]  gallstone_solid   13108 QTL population m 16-18wks N=277
Lyons2 histopathology high-fat diet number of solid gallstones   [n]  gallstone_count   13109 QTL population m 16-18wks N=277
Lyons2 histopathology high-fat diet weight of solid gallstones   [mg]  gallstone_weight   13110 QTL population m 16-18wks N=277
To see more rows use the paging control at top.

Associated genomic features
MGI-curated QTL / genomic features that have been associated with this data set:
Chol7   Chol8   Chol9   Gbvq1   Hdlq10   Hdlq13   Hdlq5   Hdlq67   Lch   Lch2   Lith12   Lith13   Lith14   Lith25   Lith6   Nhdlq1   Nhdlq2  


Project / data set:   Lyons2     a.k.a. Lyons_2003b
Excel file:   ._Data_Description_CASTx129_Lyons2003.xlsx
Excel file:   Data_Description_CASTx129_Lyons2003b.xlsx
CSV file:   ._Lyons2003_CASTx129_B37_Data.csv
CSV file:   Lyons2003b_CASTx129_B37_Data.csv
Readme / text file:   ._readme.txt
Readme / text file:   readme_Lyons2003b.txt
QC plots, figures, etc.:   QCReport_Lyons2003b.pdf