Step Procedure Equipment Data collected 1 Mice divided using split-litter design into control and endurance exercise groups - - 2 Body weight measured starting 1 wk before commencement of experiment; then weighed at 1-wk intervals until the end of testing scales body weight 3 Swim training sessions conducted 5 days a week for 5 wks Swimming arena - 4 Swimming endurance testing Swimming arena - 5 Mice enthanized, tissues harvested and assessed Dissection kit, scales, cryotome, microscope, spectrophotometer Weight of ventricles and hindlimb muscles; length of femur; muscle histology; mitochondrial enzyme activity
Evangelista FS, Brum PC, Krieger JE. Duration-controlled swimming exercise training induces cardiac hypertrophy in mice. Braz J Med Biol Res. 2003 Dec;36(12):1751-9. Epub 2003 Nov 17.