Kumar4: Gait and whole body posture measures in 52 inbred strains of mice and 10 F1 hybrid lines (2021)

Sheppard K, Gardin J, Sabnis GS, Peer A, Darrell M, Deats S, Geuther B, Lutz CM, Kumar V. Stride-level analysis of mouse open field behavior using deep-learning-based pose estimation. Cell Rep. 2022 Jan 11;38(2):110231. doi: 10.1016/j.celrep.2021.110231.   PubMed 35021077  

Kumar4 downloads
• Download Kumar4 project data set     animal data, as uploaded
• Download Kumar4 animal data matrix     with factor-related expansion applied as necessary
• Download Kumar4 strain means, SD, N, etc.     one row per strain/sex/measure
Investigators Vivek Kumar       The Jackson Laboratory,  Bar Harbor, ME
Participants Sheppard K, Gardin J, Sabnis GS, Peer A, Darrell M, Deats S, Geuther B, Lutz CM
ContactVivek Kumar     vivek.kumar@jax.org     Lab web site
Funding Provided ByNIH DA041668, DA048634; The Jackson Laboratory Directors Innovation Fund; Brain and Behavioral Foundation Young Investigator Award
Project type Phenotype strain survey data set
MPD identifiersKumar4     MPD:1102
Data changelog No updates/corrections.       Initial release date: 12/2021.
Formatted citation
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A novel method for the measurement of posture and its effect on gait, an important indicator of neural function, was studied in 52 inbred mice strains and 10 F1 hybrid lines. The basics of this toolkit is a deep convolutional neural network trained to perform pose estimation on top-down video of an open field test. This network provided twelve 2D-dimensional markers of various mouse anatomical locations, or key points, for each frame of video that described the pose of the mouse at each time point; that allowed the processing of this time series of poses and identifying poses that represent individual strides. This facilitated the extraction of several significant gait metrics, and mean, median and variance are provided for each metric.

Experimental groups in this study:
• Baseline    

Procedures conducted:
• body size and weight  Body length measurements.
• open field test  Distance traveled.
• gait analysis  Angular velocity, Stride speed, Step length, Step width, and other gait metrics.

Mice: inbred w/CC8   62 strains   ♀♂   age 10-20 wks   1 experimental group