Acclimation & testing periods
Shipment All mice were bred in animal facility of National Institute of Genetics, Japan Ages 8-12 wks of age at the start of testing Feed Given ad libitum (Rodent Diet CE-2, CLEA, Japan) Water Provided ad libitum before and during 6 different concentrations of capsaicin solutions using 1-bottle paradigm Housing After weaning, mice were grouped housed according to sex (2-5 per cage) and strain in standard sized plastic cages. One week before testing, mice were housed individually. Bedding Wood shavings Photoperiod 12 h light on, 12 h light off light-dark cycles (on at 08:00 a.m.) Humidity 40-60% Temperature 21-25 °C Health status All procedures used in this study were approved by the institutional animal care and use committee.