MPD: Data set: Karst1

Karst1: Identification of the genetic determinants for intramuscular fat content and water-holding capacity in high muscle mass mice (2011)

Kärst S, Cheng R, Schmitt AO, Yang H, de Villena FP, Palmer AA, Brockmann GA. Genetic determinants for intramuscular fat content and water-holding capacity in mice selected for high muscle mass. Mamm Genome. 2011 Oct;22(9-10):530-43. Epub 2011 Jul 6. PubMed 21732194 FullText

Intramuscular fat content and water-holding capacity are important traits in livestock. To identify genomic regions affecting them, the progeny of two inbred lines of the Berlin Muscle Mouse (BMMI816 and BMMI806), which have high muscle mass, were used as a QTL research model for livestock. These two strains were crossed reciprocally to generate F1, F2, and G3 intercross populations. Multiple related phenotypes have been measured and this is the data of G3 population. The linkage analyses with G3 animals revealed QTL on chromsome 7 and 2. For detailed protocols, see Karst et al 2011.

Note on studied population: BMMI816 x BMMI806 (Berlin Muscle Mouse)

Procedures conducted:

• study archive

Characterization of high-muscle-mass mice.

Karst1 downloads
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Use the QTL Data Downloads button near the bottom of this page.

Ontology terms mapped to Karst1 measures:

MA:0000059 blood
MA:0000155 coat hair
MA:0000672 leg muscle
MP:0000188 abnormal circulating glucose level
MP:0001259 abnormal body weight
MP:0001784 abnormal fluid regulation
MP:0002075 abnormal coat/hair pigmentation
MP:0002089 abnormal postnatal growth/weight/body size
MP:0003959 abnormal lean body mass
MP:0004043 abnormal pH regulation
MP:0004233 abnormal muscle weight
MP:0005437 abnormal glycogen level
MP:0012320 abnormal body fat mass
MP:0013403 abnormal circulating lactate level
VT:0000188 blood glucose amount
VT:0001259 body mass
VT:0001731 postnatal growth trait
VT:0001784 fluid regulation trait
VT:0004043 pH regulation trait
VT:0010463 coat/hair pigmentation trait
VT:0010482 body fat mass
VT:0010483 body lean mass
VT:0010648 muscle glycogen amount
VT:1000429 muscle lactate amount
VT:1000712 skeletal muscle mass

Investigators	B Karst Abraham A Palmer University of California, San Diego, San Diego, CA
Participants	Cheng R, Schmitt AO, Yang H, de Villena FP, Brockmann GA
Contact	Abraham A Palmer aapalmer@ucsd.edu
Affiliated Center	QTL Archive
Acknowledgements	This project was supported by the Deutsche Forschungsgemeinschaft (Project BR1285/8-1) and National Institutes of Health grant R21DA024845.
Project type	QTL Archive data set
MPD identifiers	Karst1 MPD:260
Data changelog	No updates/corrections. Initial release date: 11/2011.

QTL data set parameters:

Progenitor strains	BMMI806 BMMI816
Cross direction notation	(816 x 806) F2 x (816 x 806) F2; (806 x 816) F2 x (806 x 816) F2
Cross type	G3 reciprocal intercross
Population size	160 ♀ 172 ♂
Notes	G3 progeny

Measured phenotypes: To access these data use the QTL Data Downloads button at bottom of page.

Data set	Procedure / Protocol	Treatment	Phenotype Measure / Variable (click to view)	Panel	Sex	Age	Sample Size (Avg)
Karst1	colony observation	(none)	litter size at day of weaning (day 21) (s ='small' mice; b = 'big' mice ) WG21 26001	QTL population	both	3wks	N=166

Karst1	colony observation	(none)	coat color (white = 0, black = 1) color 26002	QTL population	both	10wks	N=166

Karst1	body weight	(none)	body weight at day 70 [g] bwd70 26003	QTL population	both	10wks	N=166

Karst1	MRI	(none)	lean mass measured by MRI(1) [g] mrilean 26004	QTL population	both	10wks	N=166

Karst1	MRI	(none)	fat mass measured by MRI [g] mrifat 26005	QTL population	both	10wks	N=166

Karst1	organ weights	(none)	muscle mass, as the sum of 2 Musculus longissimus (ml) + 2 Musculus quadriceps (mq) [g] msclemass 26006	QTL population	both	10wks	N=166

Karst1	body weight	(none)	body weight at day 70, log transformed [log(g)] logbwd70 26007	QTL population	both	10wks	N=166

Karst1	MRI	(none)	lean mass, log transformed [log(g)] logmrilean 26008	QTL population	both	10wks	N=166

Karst1	MRI	(none)	fat mass, log transformed [log(g)] logmrifat 26009	QTL population	both	10wks	N=166

Karst1	organ weights	(none)	muscle mass, log-transformed [log(g)] logmsclemass 26010	QTL population	both	10wks	N=166

Karst1	MRI	(none)	intramuscular fat content of the M. longissimus [%] imfml 26011	QTL population	both	10wks	N=166

Karst1	MRI	(none)	intramuscular fat content of the M. longissimus, log transformed [log(%)] logimfml 26012	QTL population	both	10wks	N=166

Karst1	MRI	(none)	Intramuscular fat content of the M. quadriceps [%] imfmq 26013	QTL population	both	10wks	N=166

Karst1	MRI	(none)	intramuscular fat content of the M. quadriceps, log transformed [log(%)] logimfmq 26014	QTL population	both	10wks	N=166

Karst1	centrifugation	(none)	drip loss in percent of the M. longissimus [%] dipml 26015	QTL population	both	10wks	N=166

Karst1	centrifugation	(none)	drip loss in percent of the M. longissimus, log transformed [log(%)] logdipml 26016	QTL population	both	10wks	N=166

Karst1	centrifugation	(none)	drip loss in percent of the M. quadriceps [%] dipmq 26017	QTL population	both	10wks	N=166

Karst1	centrifugation	(none)	drip loss in percent of the M. quadriceps, log transformed [log(%)] logdipmq 26018	QTL population	both	10wks	N=166

Karst1	metabolic panel	(none)	fasting blood glucose levels at day 70 [mmol] gluc70 26019	QTL population	both	10wks	N=166

Karst1	metabolic panel	(none)	fasting blood glucose levels at day 70, log transformed [log(mmol)] loggluc70 26020	QTL population	both	10wks	N=166

Karst1	pH assessment	(none)	pH value of the carcass 1 hour post mortem ph1 26021	QTL population	both	10wks	N=166

Karst1	pH assessment	(none)	pH value of the carcass 1 hour post mortem, log transformed logph1 26022	QTL population	both	10wks	N=166

Karst1	pH assessment	(none)	pH value of the carcass 24 hours post mortem ph2 26023	QTL population	both	10wks	N=166

Karst1	pH assessment	(none)	pH value of the carcass 24 hours post mortem, log transformed logph2 26024	QTL population	both	10wks	N=166

Karst1	metabolite quantification	(none)	glycogen content of the M. longissimus [mg/g] glyc 26025	QTL population	both	10wks	N=166

Karst1	metabolite quantification	(none)	glycogen content of the M. longissimus, log transformed [log(mg/g)] logglyc 26026	QTL population	both	10wks	N=166

Karst1	metabolite quantification	(none)	lactate content of the M. longissimus [mg/g] lac 26027	QTL population	both	10wks	N=166

Karst1	metabolite quantification	(none)	lactate content of the M. longissimus, log transformed [log(mg/g)] loglac 26028	QTL population	both	10wks	N=166

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Project / data set: Karst1 a.k.a. Karst_2011
Excel file: Data_Description_BMMI816x806_Karst2011.xlsx
CSV file: Karst2011_BMMI816x806_B37_Data.csv
Readme / text file: readme_Karst2011.txt
QC plots, figures, etc.: QCReport_Karst2011.pdf