Acclimation and testing periods
Ages 5-68 wks Feed All mice were fed NIH31 (6% fat) chow ad libitum Water Drinking water was acidified to pH 2.8 to 3.2 to prevent bacterial growth HousingMice were housed in cages covered with non-woven polyester filters Bedding Steam autoclaved white pine shavings Photoperiod 14 hours (light) on, 10 hours off; or 12 h light : 12 h dark cycleRelative humidity ~50% Temperature 21 °C Health status The use of animals reported herein was reviewed and approved by the Institutional Animal Care and Use Committee at each institution.