Johnson4 animal documentation

Assessment of age-dependent hearing loss in C57BL/6J-Chr#PWD/Ph/ForeJ mouse chromosome substitution strains (11-14 mo of age)   (2012)

Johnson KR, Donahue L

See also: Johnson4 protocol


Johnson4_Animal

Acclimation and testing periods

Vendor
Mice were obtained from the Repository of The Jackson Laboratory (JAX®, Bar Harbor, Maine)
Ages
Tested at 11-14 mo of age
Feed
Mice were fed pelleted and autoclaved 5K52 diet, modified from the NIH 31M open formula with 6% fat (LabDiet® 5K52 6% Fat, St. Louis, MO)
Water
Mice were provided ad libitum access to autoclaved acidified water (pH 2.8 to 3.1)
Housing
Mice were housed in micro-isolator cages from Thoren Caging Systems Inc. (Hazelton, PA). The room was supplied with HEPA-filtered air at 19 changes/h.
Bedding
Autoclaved pine shavings (Crobb Box, Ellsworth, ME)
Humidity
Relative humidity of 35% ± 4%
Photoperiod
12-h light: 12-h dark cycle
Temperature
22 ± 2°C
Regulatory compliance
All procedures were conducted in strict compliance with the policies on animal welfare of the National Institutes of Health and were approved by the Animal Care and Use Committee of The Jackson Laboratory