Jaxpheno6 animal documentation

Flow-cytometric analysis of 11 strains of mice   (2006)

Jackson Laboratory
With: Denegre J

See also: Jaxpheno6 protocol


Jaxpheno Animal Documentation

Acclimation & testing periods

Vendor
Mice were housed in JAX® production rooms until either 8 or 16 weeks of age when sample collection took place.
Ages
Mice were 10-13 wks of age on the day of sacrifice
Feed
All strains maintained on a sterilized 6% fat diet (NIH316 formula, Purina item #5K52) from age of weaning

Water
Adjusted with hydrochloric acid to pH 2.8-3.1 and supplemented with menadione sodium bisulfite (final concentration 0.4 mg/ml) to compensate for potential decrease in vitamin K activity upon autoclaving; water supplied in pinhole type bottles (Thoren Caging Systems, Inc. Hazelton, PA).
Housing
Thoren cages; Thoren Maxi Miser #3 cages used for some strains as either part of a PIV (pressurized individually ventilated) system or placed on conventional racking.
Room temperature
18 - 24°C
Bedding
Steam autoclaved white pine shavings
Relative humidity
~50%.
Photoperiod
14 hrs on (generally covering the hrs of 4:30 AM to 6:30 PM) 10 hrs off
Health status
The Jackson Laboratory Diagnostics Laboratory routinely monitors and documents animal health status using statistically valid sampling procedures and standard protocols. (Sentinel animals are tested). Detailed health status reports for each animal room represented from this project are on file and available on request. See JAX Mice Quality Control Programs or the JAX Mice Catalog for the list of agents monitored.