|Institutional authorship||Jackson Laboratory|
|Contact||Andrew J Schile firstname.lastname@example.org|
|Affiliation||JAX Mice Clinical and Research Services (JMCRS)|
|MPD identifiers||Jaxpheno20 MPD:643|
|No updates/corrections. Initial release date: 12/2018.|
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Purpose: To characterize NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ male and female mice fed a 6% fat diet (LabDiet® 5K52) between the ages of 3 and 15 weeks (males) or 24 weeks (females). Per-animal data are available in the download file.
Two hundred eighty male and two hundred eighty female mice were obtained at weaning (birthdate +/- 3 days) from seven rooms from the Bar Harbor and Sacramento facilities of The Jackson Laboratory, and weighed weekly until the oldest age held in inventory. The mice were maintained on a diet containing 6% fat (LabDiet® 5K52 formulation).
At 8, 16 and 24 weeks cohorts of 18-20 mice were weighed and body composition was analyzed using a Lunar PIXImus DEXA scanner. Calculations of body composition excluded the head. Cardiac blood was collected from at least 19 mice per age and sex under isoflurane anesthesia and divided for hematology (using a Siemens Advia 2120 or Drew Scientific Hemavet 950 FS analyzer) or serum biochemistry (Beckman AU680 analyzer). Single-cell suspensions were also analyzed by flow cytometry following a protocol found here.
Available download (per animal data): Body weights, DXA body composition, spleen flow cytometry, serum clinical chemistry, and hematology.