|Institutional authorship||Jackson Laboratory|
Andrew J Schile The Jackson Laboratory -- West, Sacramento, CA|
|Contact||Andrew J Schile firstname.lastname@example.org|
|Affiliated Center||JAX Mice Clinical and Research Services (JMCRS)|
|MPD identifiers||Jaxpheno17 MPD:625|
|No updates/corrections. Initial release date: 06/2018.|
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Purpose: To characterize BKS.Cg-Dock7m+/+Leprdb/J (db/db) mice between the ages of 4 and 20 weeks. db/+ and +/+ genotypes were also tested as controls. Mice were screened for obesity, non-fasting glucose levels, body composition, serum lipids, HbA1c (NGSP%), and splenocyte populations. Per-animal data are available in the download file.
Diet administration: All mice were fed LabDiet 5K52 formulation (6% fat by weight) from the ages of 4 to 20 weeks.
Body weight: Weekly body weights were measured from cohorts of male and female mice of all three genotypes.
Non-fasted blood glucose: Glucose levels were measured every other week for all mice. Submandibular blood glucose measurements were obtained using a OneTouch Ultra 2 or UltraMini hand-held glucometer which was validated with a control solution on each day of use. The meters had an upper limit of 600 mg/dL; any reading that exceeded this value was recorded as 600.
Body composition: Mice were analyzed using a Lunar PIXImus DEXA scanner. Calculations of body composition excluded the head. Cohorts of 10 non-fasted db/db and 4-5 db/+ and +/+ mice were tested per age and sex.
Clinical chemistry: All values were measured from serum collected from submandibular blood except HbA1c, which was measured from submandibular whole blood. Cohorts of 18-20 non-fasted db/db and 9-10 db/+ and +/+ mice were tested per age and sex. Results were obtained using a Beckman Coulter AU680 chemistry analyzer.
Spleen flow cytometry: All cell populations were calculated as percentages of the total viable splenocyte population, except for regulatory T cells which were calculated as the percentage of viable CD4+ cells. Cohorts of 10 db/db and 4-5 db/+ and +/+ mice were tested. Parameters were measured using a BD Biosciences LSR II flow cytometer using a protocol described here.
Available download (per animal data):