|Institutional authorship||Jackson Laboratory|
Andrew J Schile The Jackson Laboratory -- West, Sacramento, CA|
|Contact||Andrew J Schile email@example.com|
|Affiliated Center||JAX Mice Clinical and Research Services (JMCRS)|
|MPD identifiers||Jaxpheno15 MPD:610|
|No updates/corrections. Initial release date: 02/2018.|
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Purpose: To characterize B6.Cg-Lepob/J (ob/ob) mice between the ages of 4 and 16 weeks. Heterozygous mice (ob/+) were tested as controls. Mice were screened for obesity, non-fasting glucose levels, body composition, serum lipids, HbA1c (NGSP%), and splenocyte populations. Per-animal data are available in the download file.
Diet administration: Homozygous ob/ob mice were fed LabDiet 5K20 (10% fat by weight) and heterozygous ob/+ mice were fed LabDiet 5K52 formulation (6% fat by weight) from the ages of 4 to 16 weeks.
Body weight: At least 40 mice per genotype and sex were measured on the same day each week.
Non-fasted blood glucose: Glucose levels were determined weekly on at least 40 non-fasted mice per genotype and sex. Submandibular blood glucose measurements were obtained using a OneTouch Ultra 2 or UltraMini hand-held glucometer which was validated with a control solution on each day of use.
Body composition: Mice were analyzed using a Lunar PIXImus DEXA scanner. Calculations of body composition excluded the head.
Clinical chemistry: All values were measured from serum collected from submandibular blood except HbA1c, which was measured from submandibular whole blood. Ten to twenty non-fasted mice were tested per genotype and sex. Results were obtained using a Beckman Coulter AU680 chemistry analyzer.
Spleen flow cytometry: All cell populations were calculated as percentages of the total viable splenocyte population, except for regulatory T cells which were calculated as the percentage of viable CD4+ cells. Parameters were measured using a BD Biosciences LSR II flow cytometer using a protocol described here.
Available download (per animal data):