Mice were housed in The Jackson Laboratory (JAX®, Bar Harbor, ME) production room Breeding mice were >6 wks of age and pups are weaned at 3-4 wks of age All strains maintained on a sterilized 6% fat diet (NIH316 formula, Purina item #5K52) from age of weaning
Adjusted with hydrochloric acid to pH 2.8-3.1 and supplemented with menadione sodium bisulfite (final concentration 0.4 mg/ml) to compensate for potential decrease in vitamin K activity upon autoclaving; water supplied in pinhole type bottles (Thoren Caging Systems, Inc. Hazelton, PA). Thoren cages; Thoren Maxi Miser #3 cages used for some strains as either part of a PIV (pressurized individually ventilated) system or placed on conventional racking. 18 - 24°C Steam autoclaved white pine shavings ~50%. 14 hrs on (generally covering the hrs of 4:30 AM to 6:30 PM) 10 hrs off The Jackson Laboratory Diagnostics Laboratory routinely monitors and documents animal health status using statistically valid sampling procedures and standard protocols. (Sentinel animals are tested). Detailed health status reports for each animal room represented from this project are on file and available on request. See JAX Mice Quality Control Programs or the JAX Mice Catalog for the list of agents monitored.