Shoji H, Irino Y, Yoshida M, Miyakawa T. Behavioral effects of long-term oral administration of aluminum ammonium sulfate in male and female C57BL/6J mice.
Neuropsychopharmacol Rep. 2018 Mar;38(1):18-36. doi: 10.1002/npr2.12002. Epub 2018 Feb 9.
| Institutional authorship |
Japan Mouse Phenotypes (JMP)
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| Investigators |
Miyakawa Tsuyoshi Fujita Health University, Toyoake JAPAN |
| Participants | Shoji H, Irino Y, Yoshida M, Tsuyoshi M |
| Contact | Miyakawa Tsuyoshi miyakawa@fujita-hu.ac.jp
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| Affiliated Center | Japan Mouse Phenotypes |
| Acknowledgements | Funding provided by Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan; Food Safety Commission of Japan |
| One-strain study | |
| MPD identifiers | JMP001 MPD:648 |
| This project has not yet been released. | |
|
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Purpose: To assess effects of low dose aluminum ammonium sulfate on C57BL/6J female mice, administered at 4 wks of age to the end of testing at 37-38 wks of age. Per-animal data are available in the download file.
Methods
Mouse source and housing: Forty naive females were transported from Charles River Japan to the animal facility at Fujita Health University at the age of 25 days. After their arrival, mice were group housed (4 per cage) in plastic cages (22.7 x 32.3 x 12.7 cm) in a room with 12h light/dark cycle (lights on at 0700h). The room temperature was maintained at 23 +/- 2°C.
Aluminum ammonium sulfate administration: Mice in each cage were randomly assigned to either the vehicle-treated group (ammonium sulfate, n=20) or the low-dose aluminum ammonium sulfate group (0.075 mg/mL, n=20) (both compounds were obtained from Wako Pure Chemical Industries, Ltd). Mice were allowed to freely drink these solutions from 4 wks of age until the end of testing. Solutions were changed every 4-7 days.
Phenotypes: Body weights were measured every 2 wks, and behaviors were assessed with a battery of behavioral tests after 9 wks of age (see Table below). All mice of both experimental groups received the same test on the same day so that they underwent all of the behavioral tests in the same order. See Shoji et al., 2018 for phenotyping details.
Test Code Age at testing (wks) Weeks of treatment 1
General health and neurological screen GHNS 10 6 2
Light/dark box LD 10 6 3
Open field OF 10 6 4
Elevated plus maze EP 11 7 5
Hot plate HP 11 7 6
Social interaction SI 11 7 7
Rotarod RR 12 8 8
Three-chamber social approach CSI 14 10 9
Startle and prepulse inhibition PPI 15 11 10
Porsolt forced swim PS 15 11 11
T-maze spontaneous alternation test T-maze 16 12 12
Y-maze Y-maze 19 15 13
Barnes maze BM 26-32 22-28 14
Tail suspension TS 33 29 15
Fear conditioning FZ 33-37 29-33 16
Home-cage social interaction HCSI 37-38 33-34
Available download (per animal data):
JMP001.xlsx