JMP001: Behavioral effects of low dose oral administration of aluminum ammonium sulfate in female C57BL/6J mice (2019) [Pending]

Shoji H, Irino Y, Yoshida M, Miyakawa T. Behavioral effects of long-term oral administration of aluminum ammonium sulfate in male and female C57BL/6J mice. Neuropsychopharmacol Rep. 2018 Mar;38(1):18-36. doi: 10.1002/npr2.12002. Epub 2018 Feb 9.   PubMed 30106265  

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Institutional authorship Japan Mouse Phenotypes (JMP)     Lab web site
Investigators Miyakawa Tsuyoshi       Fujita Health University,  Toyoake JAPAN
Participants Shoji H, Irino Y, Yoshida M, Tsuyoshi M
ContactMiyakawa Tsuyoshi     Lab web site
Affiliated CenterJapan Mouse Phenotypes
AcknowledgementsFunding provided by Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan; Food Safety Commission of Japan
Project type One-strain study
MPD identifiersJMP001     MPD:648
Data changelog This project has not yet been released.
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Purpose: To assess effects of low dose aluminum ammonium sulfate on C57BL/6J female mice, administered at 4 wks of age to the end of testing at 37-38 wks of age. Per-animal data are available in the download file.


Mouse source and housing: Forty naive females were transported from Charles River Japan to the animal facility at Fujita Health University at the age of 25 days. After their arrival, mice were group housed (4 per cage) in plastic cages (22.7 x 32.3 x 12.7 cm) in a room with 12h light/dark cycle (lights on at 0700h). The room temperature was maintained at 23 +/- 2°C.

Aluminum ammonium sulfate administration: Mice in each cage were randomly assigned to either the vehicle-treated group (ammonium sulfate, n=20) or the low-dose aluminum ammonium sulfate group (0.075 mg/mL, n=20) (both compounds were obtained from Wako Pure Chemical Industries, Ltd). Mice were allowed to freely drink these solutions from 4 wks of age until the end of testing. Solutions were changed every 4-7 days.

Phenotypes: Body weights were measured every 2 wks, and behaviors were assessed with a battery of behavioral tests after 9 wks of age (see Table below). All mice of both experimental groups received the same test on the same day so that they underwent all of the behavioral tests in the same order. See Shoji et al., 2018 for phenotyping details.


Test Code Age at testing (wks) Weeks of treatment


General health and neurological screen GHNS 10 6


Light/dark box LD 10 6


Open field OF 10 6


Elevated plus maze EP 11 7


Hot plate HP 11 7


Social interaction SI 11 7


Rotarod RR 12 8


Three-chamber social approach CSI 14 10


Startle and prepulse inhibition PPI 15 11


Porsolt forced swim PS 15 11


T-maze spontaneous alternation test T-maze 16 12


Y-maze Y-maze 19 15


Barnes maze BM 26-32 22-28


Tail suspension TS 33 29


Fear conditioning FZ 33-37 29-33


Home-cage social interaction HCSI 37-38 33-34


Available download (per animal data):