Step Procedure Data collected * 1 Body composition See HMDPpheno2 2 Following 16h fast, complete blood count and plasma lipids determined See HMDPpheno5 and HMDPpheno2 3 Mice euthanized, tissues harvested - 4 Liver metabolic profiling Metabolite relative abundance
*Supplementary data are available for this project.
Mice shipped at 6-10 wks of age, tested at 16 wks of age.
Liver samples are analyzed at Metabolon (Durham NC); profiling complete in 6 days. The non-targeted metabolic profiling instrumentation employed combines three independent platforms: ultrahigh performance liquid chromatography/tandem mass spectrometry (UHPLC/MS/MS2) optimized for basic species, UHPLC/MS/MS2 optimized for acidic species, and gas chromatography/mass spectrometry (GC/MS). Three types of controls are analyzed in concert: aliquots of a pooled sample derived from a portion of all experimental samples (technical replicates), extracted water samples serve as process blanks, and a cocktail of standards spiked into every analyzed sample allow instrument performance monitoring. Experimental samples and controls are randomized across platform run days. Data are corrected by registering the median of each run-day to one followed by normalizing each data point proportionately.
UHPLC/MS/MS2 is abbreviated to HPLC/MS (for methodology) on project pages.
*Supplementary data (Z scores) are available for this project.
Evans AM, DeHaven CD, Barrett T, Mitchell M, Milgram E. Integrated, nontargeted ultrahigh performance liquid chromatography/electrospray ionization tandem mass spectrometry platform for the identification and relative quantification of the small-molecule complement of biological systems. Anal Chem. 2009 Aug 15;81(16):6656-67. doi: 10.1021/ac901536h.
Ohta T, Masutomi N, Tsutsui N, Sakairi T, Mitchell M, Milburn MV, Ryals JA, Beebe KD, Guo L. Untargeted metabolomic profiling as an evaluative tool of fenofibrate-induced toxicology in Fischer 344 male rats. Toxicol Pathol. 2009 Jun;37(4):521-35. doi: 10.1177/0192623309336152.