Hematological parameters in males of 83 strains of mice in the Hybrid Mouse Diversity Panel (HMDP) (2013)
Davis RC, van Nas A, Bennett BJ, Eskin E, Lusis AJ
With: Orozco L, Pan C, Rau CD
HMDPpheno5
Protocol
Project
protocol
-
Contents
Workflow
and
sampling
Step |
Procedure |
Equipment |
Data
collected |
1 |
Mice
fasted
4-5
h |
- |
- |
2 |
Mice
anesthetized |
- |
- |
| 3 |
Blood
collected
from
retro-orbital
plexus |
Capillary
tubes |
- |
| 4 |
Complete
blood
cell
profiling |
Hematology
analyzer |
Cell
counts,
RBC
parameters,
hemoglobin,
hematocrit,
platelet
counts
and
volume |
Equipment
and
supplies
- EDTA-coated
glass
capillary
tubes
- HemaTrue
Veterinary
Hematology
Analyzer
(Heska,
Loveland
CO)
Reagents
and
solutions
- See
instructions
for
HemaTrue
Veterinary
Hematology
Analyzer
Acclimation
Mice
shipped
from
vendor
at
6-10
wks
of
age
and
acclimated
for
several
weeks;
tested
at
16
wks.
Procedure:
Hematology
- Mice
fasted
4-5
h.
- Mice
anesthetized
2-3
h
after
beginning
of
the
light
cycle.
- Blood
collected
from
retro-orbital
plexus
into
EDTA-coated
glass
capillary
tubes.
- Samples
analyzed
using
HemaTrue
Veterinary
Hematology
Analyzer
following
manufacturer
instructions.
Data
collected
by
investigator
- complete
blood
counts
- RBC
parameters
- hemaglobin
- hematocrit
- platelet
count
and
volume