Plasmodium
chabaudi
chabaudi
AS:
a
lactate
dehydrogenase
virus-free
isolate
originally
obtained
from
Dr.
Walliker
(University
of
Edinburgh).
The
parasite
is
maintained
by
weekly
passage
in
A/J
mice
by
intraperitoneal
infection
with
106
parasitized
red
blood
cells
(pRBCs)
suspended
in
1
mL
of
pyrogen-free
saline.
Experimental
mice
are
infected
with
106
pRBCs
intraperitoneally.
Procedure:
Monitoring
parasitemia
and
survival
The
percentage
of
pRBCs
is
determined
daily
on
thin
blood
smears
stained
with
Diff-Quick
on
days
4-21
after
infection.
Survival
is
monitored
twice
daily
and
moribund
animals
are
euthanized.