GMC21 project protocol
Tibia length and heart, liver, and spleen weights in 8 inbred founder strains of the Collaborative Cross (2020)
German Mouse Clinic and Department of Infection Genetics, HZI
With: Hrabě de Angelis M, Fuchs H, Gailus-Durner V, Neff F, Calzada-Wack J, Höfler H, Lengger C, Kollmus H, Schughart K
See also: GMC21 animal documentation
German Mouse Clinic: Phenotyping Pipeline
Organ weights and tibia length: 20-21 wks of age
||Heart, liver, spleen weights
- CO2 chamber
- Dissection kit
- Digital caliper
- CO2 gas
- 80% ethanol
- 4% neutral-buffered formalin
Procedure: Organ weights and tibia length
- Mice are euthanized in the CO2 chamber.
- Body weight is measured.
- The abdomen of each mouse is doused with ethanol to avoid hair contamination.
- The mouse is pinned down on a cork board ventral-side-up.
- The fur is opened above the symphysis with a small incision; a double-Y is cut through the skin without disturbing the underlying abdominal muscles; the Y-incision is extended to the mandibular bone and toward the paws.
- Skin flaps are pinned back onto the board, without disturbing abdominal muscles.
- The abdominal wall is opened with an incision above the pelvis in a T-manner to the ribcage and sternal bone.
- The abdominal cavity is opened by folding the muscles back.
- Organs are dissected:
- Heart: dissect from aortic root right above the atria; dip the heart onto tissue to remove as much blood from the atria and chambers as possible.
- Liver: dissect from the stomach, diaphragm ligaments, and vessels at the hilus.
- Spleen: dissect carefully from the pancreas; remove vessels at the hilus.
- Organs are weighed.
- Tibia length is measured by removing the pin from the left hindpaw, flexing the limb and measuring the length with caliper from the patella to the ankle.
- Body weight
- Heart weight
- Liver weight
- Spleen weight