GMC19 project protocol
Lung function of females of 8 inbred founder strains of the Collaborative Cross (2020)
German Mouse Clinic and Department of Infection Genetics, HZI
With: Hrabě de Angelis M, Fuchs H, Gailus-Durner V, Stöger T, Yildirim AÖ, Eickelberg O, Lengger C, Kollmus H, Schughart K
GMC19 animal documentation
German Mouse Clinic: Phenotyping Pipeline
Lung function: 21-22 wks of age
Project
protocol
-
Contents
Workflow
and
sampling
Step
Procedure
Equipment
Data
collected
1
Body weight
Balance
Body weight
2
Mice
anesthetized
-
-
3
Lung function
Pulmonary function device
Lung volume, mechanics, compliance, airway resistance
Equipment
and
supplies
Balance: Ohaus (AV213C)
18-gauge cannula
Pulmonary function device: Buxco Research Systems (Fine-Pointe RC System), Wilmington NC
Pulmonary function device: Buxco Research Systems (Forced Maneuver System), Wilmington NC
Reagents
and
solutions
Ketamine
Xylazine
Cleaning solution
Procedure: Lung function
Mice are weighed and anesthetized by i.p. injection of ketamine (137 mg/kg BW) and xylazine (6.6 mg/kg BW) (performed in the morning).
The trachea is opened by a small incision and a cannula is inserted and fixated by ligation.
Each mouse is placed in the Fine-Pointe System (heated plethysmograph chamber).
Mice are ventilated at an average rate of 130 breaths per min, and flow and mouth pressure and heart rate are monitored.
After an initial acclimation period of three minutes, a two-minute measurement is performed for resistance and compliance.
Mice are then transferred to the Forced Maneuver System where the rest of the pulmonary measurements are taken.
Data
collected
by
investigator
Body weight
Chord compliance
Dynamic lung compliance
Expiratory reverse volume
Forced expiratory volume
Functional residual capacity
Forced vital capacity
Inspiratory capacity
Peak expiratory flow
Lung resistance
Residual volume
Total lung capacity
Tidal volume
Vital capacity