GMC19 project protocol
Lung function of females of 8 inbred founder strains of the Collaborative Cross (2020)
German Mouse Clinic and Department of Infection Genetics, HZI
With: Hrabě de Angelis M, Fuchs H, Gailus-Durner V, Stöger T, Yildirim AÖ, Eickelberg O, Lengger C, Kollmus H, Schughart K
See also: GMC19 animal documentation
German Mouse Clinic: Phenotyping Pipeline
Lung function: 21-22 wks of age
||Pulmonary function device
||Lung volume, mechanics, compliance, airway resistance
- Balance: Ohaus (AV213C)
- 18-gauge cannula
- Pulmonary function device: Buxco Research Systems (Fine-Pointe RC System), Wilmington NC
- Pulmonary function device: Buxco Research Systems (Forced Maneuver System), Wilmington NC
- Cleaning solution
Procedure: Lung function
- Mice are weighed and anesthetized by i.p. injection of ketamine (137 mg/kg BW) and xylazine (6.6 mg/kg BW) (performed in the morning).
- The trachea is opened by a small incision and a cannula is inserted and fixated by ligation.
- Each mouse is placed in the Fine-Pointe System (heated plethysmograph chamber).
- Mice are ventilated at an average rate of 130 breaths per min, and flow and mouth pressure and heart rate are monitored.
- After an initial acclimation period of three minutes, a two-minute measurement is performed for resistance and compliance.
- Mice are then transferred to the Forced Maneuver System where the rest of the pulmonary measurements are taken.
- Body weight
- Chord compliance
- Dynamic lung compliance
- Expiratory reverse volume
- Forced expiratory volume
- Functional residual capacity
- Forced vital capacity
- Inspiratory capacity
- Peak expiratory flow
- Lung resistance
- Residual volume
- Total lung capacity
- Tidal volume
- Vital capacity