GMC10 project protocol

Body composition assessed by NMR at two ages in 8 inbred founder strains of the Collaborative Cross   (2020)

German Mouse Clinic and Department of Infection Genetics, HZI
With: Hrabě de Angelis M, Fuchs H, Gailus-Durner V, Rozman J, Klingenspor M, Lengger C, Kollmus H, Schughart K

See also: GMC10 animal documentation


German Mouse Clinic: Phenotyping Pipeline

Determination of body composition by NMR: 12-13 and 18-19 wks of age

 

  • Project protocol - Contents

    Workflow and sampling

    Step
    Procedure
    Equipment
    Data collected
    1 Weigh mice Balance Body weight
    2
    Determination of body composition Body composition analyzer (NMR)
    Fat tissue mass, lean tissue mass

    Equipment and supplies

    • Balance: Sartorius (MSE 2202 S-100 D0)
    • Whole body composition analyzer (NMR): Bruker (MiniSpec LF50)

    Reagents and solutions

    • Cleaning solution

    Procedure: Body composition measured by NMR

      1. Mice are first weighed using a balance.
      2. Mice are placed in the analyzer and relevant parameters are measured (whole body with head).
      3. Mice are returned to their homecage.

    Data collected by investigator

    • Body weight
    • Fat tissue mass
    • Lean tissue mass