Thermal nociception assessed by the hot plate test in 8 inbred founder strains of the Collaborative Cross (2020)
German Mouse Clinic and Department of Infection Genetics, HZI
With: Hrabě de Angelis M, Fuchs H, Gailus-Durner V, Racz I, Becker L, Zimmer A, Lengger C, Kollmus H, Schughart K
Plexiglas wall: Circular, 28-cm diameter, 20-cm high
Stopwatch
Reagents
and
solutions
Cleaning solution
Procedure: Hot plate test
Mice are individually placed within the Plexiglas walls which rests upright on the hot plate (metal surface maintained at 52 +/- 0.2 °C); all four paws are in contact with the metal surface.
Mice remain on the surface until they show first and second reactions to the hotplate regarded indicative of nociception pathway function: hindpaw lick, hindpaw shake/flutter, jumping, or none.
Cutoff time is 30 s.
After the first two reactions or latest cutoff time, mice are removed promptly and placed in their homecage.
Latency is recorded to the nearest 0.1 s.
Data
collected
by
investigator
Body weight
Latency to respond to hotplate: first two reactions (hindpaw lick, hindpaw shake/flutter, jumping, or none)