GMC07 project protocol
Thermal nociception assessed by the hot plate test in 8 inbred founder strains of the Collaborative Cross (2020)
German Mouse Clinic and Department of Infection Genetics, HZI
With: Hrabě de Angelis M, Fuchs H, Gailus-Durner V, Racz I, Becker L, Zimmer A, Lengger C, Kollmus H, Schughart K
German Mouse Clinic: Phenotyping Pipeline
Hot plate test (nociceptive pain): 11 - 12 wks of age
||Thermal nociception test
||Latency to respond, type of response
- Hot plate: TSE GmbH (Hot Plate 602001)
- Plexiglas wall: Circular, 28-cm diameter, 20-cm high
Procedure: Hot plate test
- Mice are individually placed within the Plexiglas walls which rests upright on the hot plate (metal surface maintained at 52 +/- 0.2 °C); all four paws are in contact with the metal surface.
- Mice remain on the surface until they show first and second reactions to the hotplate regarded indicative of nociception pathway function: hindpaw lick, hindpaw shake/flutter, jumping, or none.
- Cutoff time is 30 s.
- After the first two reactions or latest cutoff time, mice are removed promptly and placed in their homecage.
- Latency is recorded to the nearest 0.1 s.
- Body weight
- Latency to respond to hotplate: first two reactions (hindpaw lick, hindpaw shake/flutter, jumping, or none)
- Type of first and second reaction