Acclimation and testing periods
Vendor Mice were obtained at 6 wks of age from The Jackson Laboratory JAX® (Bar Harbor, ME) Ages 6-8 wks Duration 1-3 weeks in conventional, non-barrier facility at VA Medical Center, Portland Oregon USA Feed Purina 5001 bought locally; ad libitum Water Tap; ad libitum, except when ethanol was substituted for water Housing4 mice per cage, separated by sex and strain in clear polycarbonate/ polysulfone cages (28 x 18 x 13 cm) Bedding 1/4 inch Bed-o-cob Photoperiod 12 h on (0600 h) 12 h off ; lights on at 0600 Temperature 21 ± 2.0°C Health status Mice were reported in excellent health