Donahue19 animal documentation

Gestation duration and maternal weight gain in C57BL/6J-Chr#A/J/NaJ mouse chromosome substitution strains   (2010)

Donahue L

See also: Donahue19 protocol


Donahue19_Animal

Acclimation and testing periods

Vendor
B6.A consomic mice were obtained from the Repository of The Jackson Laboratory (JAX®, Bar Harbor, Maine)
Ages
Mice were tested 6-8 wks of age
Feed
Mice were fed ad libitum pelleted and autoclaved diet of NIH 31M formula with 6% fat (Purina Mills Inc., Richmond, IN)
Water
Mice were provided ad libitum access to autoclaved acidified water (pH 2.8 to 3.1)
Housing
Mice were housed in groups of 4-5 mice in individually ventilated micro-isolator cages from Thoren Caging Systems Inc. (Hazelton, PA) within an SPF barrier room. Each pen contained a wire-rod metal top that holds feed pellets and a water bottle. The room was supplied with HEPA-filtered air at 19 changes/h.
Bedding
Autoclaved white pine shavings (Crobb Box, Ellsworth, ME)
Humidity
Relative humidity of 35% ± 4%
Photoperiod
12-h light: 12-h dark cycle
Temperature
22 ± 2°C
Regulatory compliance
All animal experiments have been conducted according to relevant national and international guidelines (AALAC and IACUC) and have been approved by the Jackson Laboratory Animal Care and Use Committee (Genetic Resource Science protocol #99066)