MPD: Donahue18: animal documentation

Donahue18 animal documentation

Gestation duration and maternal weight gain in 15 inbred mouse strains (2010)

Donahue L
With: Murray SA, Morgan JL, Kane C, Sharma Y, Heffner CS, Lake J

Donahue19_Animal

Acclimation and testing periods

Vendor

Inbred mice were obtained from the Repository of The Jackson Laboratory (JAX®, Bar Harbor, Maine)

Ages

Mice were tested 6-8 wks of age

Feed

Mice were fed ad libitum pelleted and autoclaved diet of NIH 31M formula with 6% fat (Purina Mills Inc., Richmond, IN)

Water

Mice were provided ad libitum access to autoclaved acidified water (pH 2.8 to 3.1)

Housing
Mice were housed in groups of 4-5 mice in individually ventilated micro-isolator cages from Thoren Caging Systems Inc. (Hazelton, PA) within an SPF barrier room. Each pen contained a wire-rod metal top that holds feed pellets and a water bottle. The room was supplied with HEPA-filtered air at 19 changes/h.

Bedding

Autoclaved white pine shavings (Crobb Box, Ellsworth, ME)

Humidity

Relative humidity of 35% ± 4%

Photoperiod

12-h light: 12-h dark cycle

Temperature

22 ± 2°C

Regulatory compliance

All animal experiments have been conducted according to relevant national and international guidelines (AALAC and IACUC) and have been approved by the Jackson Laboratory Animal Care and Use Committee (Genetic Resource Science protocol #99066)

Vendor	Inbred mice were obtained from the Repository of The Jackson Laboratory (JAX®, Bar Harbor, Maine)
Ages	Mice were tested 6-8 wks of age
Feed	Mice were fed ad libitum pelleted and autoclaved diet of NIH 31M formula with 6% fat (Purina Mills Inc., Richmond, IN)
Water	Mice were provided ad libitum access to autoclaved acidified water (pH 2.8 to 3.1)
Housing	Mice were housed in groups of 4-5 mice in individually ventilated micro-isolator cages from Thoren Caging Systems Inc. (Hazelton, PA) within an SPF barrier room. Each pen contained a wire-rod metal top that holds feed pellets and a water bottle. The room was supplied with HEPA-filtered air at 19 changes/h.
Bedding	Autoclaved white pine shavings (Crobb Box, Ellsworth, ME)
Humidity	Relative humidity of 35% ± 4%
Photoperiod	12-h light: 12-h dark cycle
Temperature	22 ± 2°C
Regulatory compliance	All animal experiments have been conducted according to relevant national and international guidelines (AALAC and IACUC) and have been approved by the Jackson Laboratory Animal Care and Use Committee (Genetic Resource Science protocol #99066)