Donahue10 animal documentation

Reproductive performance of C57BL/6J-Chr#A/J/NaJ mouse chromosome substitution strains   (2011)

Donahue L

See also: Donahue10 protocol


Donahue10_Animal

Acclimation and testing periods

Supplier
B6.A mice were obtained from the repository of The Jackson Laboratory (JAX®, Bar Harbor, Maine)
Ages
All mice were pair-mated to siblings between 3 to 13 wks of age, and retired from breeding between 19 to 53 wks of age
Feed
Pelleted and autoclaved 5K52 diet, modified from the NIH 31M open formula with 6% fat (LabDiet® 5K52 6% Fat, St. Louis, MO), was provided ad libitum
Water
Access to autoclaved acidified water (pH 2.8 to 3.1) was provided ad libitum
Housing
Mice were housed in individually ventilated micro-isolator cages from Thoren Caging Systems Inc. (Hazelton, PA) within an SPF barrier room. The room was supplied with HEPA-filtered air at 19 changes/h.
Bedding
Autoclaved pine shavings (Crobb Box, Ellsworth, ME)
Humidity
35 ± 4%
Photoperiod
12-h light: 12-h dark cycle
Temperature
22 ± 2°C
Regulatory compliance
All procedures were conducted in strict compliance with the policies on animal welfare of the National Institutes of Health and were approved by the Animal Care and Use Committee of The Jackson Laboratory