DiPetrillo2 animal documentation

Urine albumin and creatinine in males of 7 inbred founder strains and 150+ emerging lines (pre-CC) of the Collaborative Cross   (2012)

DiPetrillo K
With: Thaisz J, Tsaih SW, Feng M, Philip VM, Zhang Y, Yanas L, Sheehan S, Xu L, Miller DR, Paigen B, Chesler EJ, Churchill GA

See also: DiPetrillo2 protocol


DiPetrillo2_Animal

Acclimation and testing periods

Vendor
Male inbred mice were obtained The Jackson Laboratory (JAX®, Bar Harbor, Maine). Collaborative Cross male mice obtained from Oak Ridge National Laboratory (Oak Ridge, TN 37831).
Ages
Mice were tested 10 wks of age
Feed
Mice were fed ad libitum pelleted and autoclaved diet of NIH 31M formula with 6% fat (Purina Mills Inc., Richmond, IN)
Water
Mice were provided ad libitum access to autoclaved acidified water (pH 2.8 to 3.1)
Housing
Mice were housed in groups of 4-5 mice in individually ventilated micro-isolator cages from Thoren Caging Systems Inc. (Hazelton, PA) within an SPF barrier room. Each pen contained a wire-rod metal top that holds feed pellets and a water bottle. The room was supplied with HEPA-filtered air at 19 changes/h.
Bedding
Autoclaved white pine shavings (Crobb Box, Ellsworth, ME)
Humidity
Relative humidity of 35% ± 4%
Photoperiod
12-h light: 12-h dark cycle
Temperature
22 ± 2°C
Regulatory compliance
All animal experiments have been conducted according to relevant national and international guidelines (AALAC and IACUC) and have been approved by the Jackson Laboratory Animal Care and Use Committee