DiPetrillo2 animal documentation

Urine albumin and creatinine in males of 7 inbred founder strains and 150+ emerging lines (pre-CC) of the Collaborative Cross   (2012)

Acclimation and testing periods

Vendor	Male inbred mice were obtained The Jackson Laboratory (JAX®, Bar Harbor, Maine). Collaborative Cross male mice obtained from Oak Ridge National Laboratory (Oak Ridge, TN 37831).
Ages	Mice were tested 10 wks of age
Feed	Mice were fed ad libitum pelleted and autoclaved diet of NIH 31M formula with 6% fat (Purina Mills Inc., Richmond, IN)
Water	Mice were provided ad libitum access to autoclaved acidified water (pH 2.8 to 3.1)
Housing	Mice were housed in groups of 4-5 mice in individually ventilated micro-isolator cages from Thoren Caging Systems Inc. (Hazelton, PA) within an SPF barrier room. Each pen contained a wire-rod metal top that holds feed pellets and a water bottle. The room was supplied with HEPA-filtered air at 19 changes/h.
Bedding	Autoclaved white pine shavings (Crobb Box, Ellsworth, ME)
Humidity	Relative humidity of 35% ± 4%
Photoperiod	12-h light: 12-h dark cycle
Temperature	22 ± 2°C
Regulatory compliance	All animal experiments have been conducted according to relevant national and international guidelines (AALAC and IACUC) and have been approved by the Jackson Laboratory Animal Care and Use Committee