Individual variation in sensitivity to acute ethanol (EtOH) challenge is associated with alcohol drinking and abuse. Inbred strain C57BL/6J (B6) and 129S1/SvImJ (S1) showed different responses on certain measures of acute EtOH intoxication. To gain insight into genetic factors contributing to these differences, a QTL analysis of EtOH traits was performed in B6x129S1 F2 population. The result revealed several additive and interacting loci, and the QTLs for different EtOH phenotypes were largely non-overlapping. For detailed protocols, see Chesler et al 2012.