Brockmann1: Genetic factors of body, muscle, fat weight and their pleiotropic effects in F2 progeny from 2 intercross populations (2009)

Brockmann GA, Tsaih SW, Neuschl C, Churchill GA, Li R. Genetic factors contributing to obesity and body weight can act through mechanisms affecting muscle weight, fat weight, or both. Physiol Genomics. 2009 Jan 8;36(2):114-26. Epub 2008 Nov 4.   PubMed 18984673     FullText


       
This project is to investigate the relationships between genetic loci and different phenotypes influencing body weight. Two F2 populations were generated: Cross 1 is an intercross of DBA/2 and NMRI8; Cross 2 is an intercross of DBA/2 and DU6i. For both crosses the F2 progeny were fed breeding diet (1) and phenotyped for body weight, muscle weight, fat weight and other related phenotypes (studied phenotypes vary in cross1 vs. cross2). For detailed protocols see Brockmann et al 2009.

(1) Breeding diet: containing 12.5 MJ/kg metabolic energy with an average content of 22.5% crude protein, 5.0% crude fat, 4.5% crude fiber, 6.5% crude ash, 13.5% water, 48.0% N-free extract, vitamins, trace elements, amino acids, and minerals (Altromin diet 1314).

Note on studied population: DBA2J x NMRI8 and DBA2J x DU6i intercrosses

Procedures conducted:
• study archive  Body, organ weights, litter size, abdominal fat, lipids, insulin, leptin.

Brockmann1 downloads
QTL projects have several available downloads.
Use the QTL Data Downloads button near the bottom of this page.
Ontology terms mapped to Brockmann1 measures:
  MA:0000059   blood
  MA:0000141   spleen
  MA:0000326   reproductive system
  MA:0000358   liver
  MA:0000368   kidney
  MA:0000472   abdominal fat pad
  MA:0002363   quadriceps
  MA:0002501   plasma
  MA:0002502   serum
  MP:0000010   abnormal abdominal fat pad morphology
  MP:0000180   abnormal circulating cholesterol level
  MP:0000184   abnormal circulating HDL cholesterol level
  MP:0001259   abnormal body weight
  MP:0001560   abnormal circulating insulin level
  MP:0001933   abnormal litter size
  MP:0002707   abnormal kidney weight
  MP:0004700   abnormal circulating insulin-like growth factor I level
  MP:0004847   abnormal liver weight
  MP:0004951   abnormal spleen weight
  MP:0005667   abnormal circulating leptin level
  MP:0011969   abnormal circulating triglyceride level
  MP:0020164   abnormal quadriceps weight
  VT:0000010   abdominal fat pad morphology trait
  VT:0000180   blood cholesterol amount
  VT:0000184   blood HDL cholesterol amount
  VT:0001259   body mass
  VT:0001560   blood insulin amount
  VT:0001923   female fertility trait
  VT:0002161   female fecundity trait
  VT:0002644   blood triglyceride amount
  VT:0002707   kidney mass
  VT:0003402   liver mass
  VT:0004951   spleen mass
  VT:0005667   blood leptin amount
  VT:0010479   blood insulin-like growth factor amount
  VT:0010569   quadriceps morphology trait
  VT:1000189   offspring quantity

Investigators G A Brockmann
Gary A Churchill       The Jackson Laboratory,  Bar Harbor, ME
Participants Tsaih SW, Neuschl C, Li R
ContactGary A Churchill     gary.churchill@jax.org
Affiliated CenterQTL Archive
AcknowledgementsThe project was supported from the Federal Ministry for Education and Research, Germany (NGFN project 01GS0486, G. A. Brockmann),Deutsche Forschungsgemeinschaft (GRK 1208, C. Neuschl), and National Institute of General Medical Sciences (GM-076468, G. A. Churchill).
Project type QTL Archive data set
MPD identifiersBrockmann1     MPD:213
Data changelog No updates/corrections.       Initial release date: 09/2010.
QTL data set parameters:
Cross 1: DBA/2 x NMRI8       Brockmann1
Progenitor strains DBA/2J     NMRI8    
Cross direction notation(DBA/2 x NMRI8) F1 x (DBA/2 x NMRI8) F1
Cross typeF2 intercross
Population size 142 ♀     133 ♂

Progenitor strains DBA/2J     DU6i    
Cross typeNone
Population size None ♀     None ♂


Measured phenotypes:       To access these data use the QTL Data Downloads button at bottom of page.
Select table page: First Prev 1 - 10 / 29 (29) Next Last
Data set
Procedure / Protocol
Treatment
Phenotype Measure / Variable (click to view)
Panel
No. of Strains
Sex
Age
Sample Size (Avg)
Brockmann1 colony observation (none) number of offspring per litter: 3 classes   [n]  pupsize   21301 QTL population both 6wks N=138
Brockmann1 body weight (none) mouse body weight at week 3   [g]  Bw3   21302 QTL population both 3wks N=138
Brockmann1 body weight (none) mouse body weight at week 4   [g]  Bw4   21303 QTL population both 4wks N=138
Brockmann1 body weight (none) mouse body weight at week 5   [g]  Bw5   21304 QTL population both 5wks N=138
Brockmann1 body weight (none) mouse body weight at week 6   [g]  Bw6   21305 QTL population both 6wks N=138
Brockmann1 organ weights (none) abdominal fat   [mg]  Afw   21306 QTL population both 6wks N=138
Brockmann1 organ weights (none) abdominal fat percentage = %(Afw/body weight)   [%]  Afp   21307 QTL population both 6wks N=138
Brockmann1 muscle weights (none) muscle weight   [mg]  Mw   21308 QTL population both 6wks N=138
Brockmann1 organ weights (none) weight of liver   [mg]  Liver   21309 QTL population both 6wks N=138
Brockmann1 organ weights (none) weight of kidney   [mg]  Kidney   21310 QTL population both 6wks N=138
To see more rows use the paging control at top.



Cross 1: DBA/2 x NMRI8     Brockmann1     a.k.a. Brockmann_2009a
Excel file:   Data_Description_DBA2xNMRI8_Brockmann2009.xlsx
CSV file:   Brockmann2009_DBA2xNMRI8_B37_Data.csv
Readme / text file:   ._readme.txt
Readme / text file:   readme_Brockmann2009a.txt
QC plots, figures, etc.:   QCReport_Brockmann2009a.pdf

Project / data set:   Brockmann1b     a.k.a. None

QTL file list not available on this MPD instance.