MPD: Data set: Brockmann1

Brockmann1: Genetic factors of body, muscle, fat weight and their pleiotropic effects in F2 progeny from 2 intercross populations (2009)

Brockmann GA, Tsaih SW, Neuschl C, Churchill GA, Li R. Genetic factors contributing to obesity and body weight can act through mechanisms affecting muscle weight, fat weight, or both. Physiol Genomics. 2009 Jan 8;36(2):114-26. Epub 2008 Nov 4. PubMed 18984673 FullText

Brockmann1 downloads
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Use the QTL Data Downloads button near the bottom of this page.

Participants	G A Brockmann Gary A Churchill The Jackson Laboratory Bar Harbor, ME With: Tsaih SW, Neuschl C, Li R
Contact	Gary A Churchill gary.churchill@jax.org
Affiliation	Churchill Group QTL Archive
Acknowledgements	The project was supported from the Federal Ministry for Education and Research, Germany (NGFN project 01GS0486, G. A. Brockmann),Deutsche Forschungsgemeinschaft (GRK 1208, C. Neuschl), and National Institute of General Medical Sciences (GM-076468, G. A. Churchill).
Project type	QTL Archive data set
MPD identifiers	Brockmann1 MPD:213
Data changelog	No updates/corrections. Initial release date: 09/2010.

This project is to investigate the relationships between genetic loci and different phenotypes influencing body weight. Two F2 populations were generated: Cross 1 is an intercross of DBA/2 and NMRI8; Cross 2 is an intercross of DBA/2 and DU6i. For both crosses the F2 progeny were fed breeding diet (1) and phenotyped for body weight, muscle weight, fat weight and other related phenotypes (studied phenotypes vary in cross1 vs. cross2). For detailed protocols see Brockmann et al 2009.

(1) Breeding diet: containing 12.5 MJ/kg metabolic energy with an average content of 22.5% crude protein, 5.0% crude fat, 4.5% crude fiber, 6.5% crude ash, 13.5% water, 48.0% N-free extract, vitamins, trace elements, amino acids, and minerals (Altromin diet 1314).

QTL data set parameters:

Cross 1: DBA/2 x NMRI8 Brockmann1
Progenitor strains	DBA/2J NMRI8
Cross direction notation	(DBA/2 x NMRI8) F1 x (DBA/2 x NMRI8) F1
Cross type	F2 intercross
Population size	142 ♀ 133 ♂

Cross 2: DBA/2 x DU6i Brockmann1b
Progenitor strains	DBA/2J DU6i
Cross direction notation	(DBA/2 x DU6i) F1 x (DBA/2 x DU6i) F1
Cross type	F2 intercross
Population size	174 ♀ 228 ♂

Measured phenotypes: To access these data use the QTL Data Downloads button at bottom of page.

Data set	MPD ID	Procedure / Protocol	Drug or Challenge	Phenotype Measure	Panel	Sex	Age	Sample Size (Avg)
Brockmann1	21301	colony observation	(none)	number of offspring per litter: 3 classes [n] pupsize	QTL population	both	6wks	N=138

Brockmann1	21302	body weight	(none)	mouse body weight at week 3 [g] Bw3	QTL population	both	3wks	N=138

Brockmann1	21303	body weight	(none)	mouse body weight at week 4 [g] Bw4	QTL population	both	4wks	N=138

Brockmann1	21304	body weight	(none)	mouse body weight at week 5 [g] Bw5	QTL population	both	5wks	N=138

Brockmann1	21305	body weight	(none)	mouse body weight at week 6 [g] Bw6	QTL population	both	6wks	N=138

Brockmann1	21306	organ weights	(none)	abdominal fat [mg] Afw	QTL population	both	6wks	N=138

Brockmann1	21307	organ weights	(none)	abdominal fat percentage = %(Afw/body weight) [%] Afp	QTL population	both	6wks	N=138

Brockmann1	21308	muscle weights	(none)	muscle weight [mg] Mw	QTL population	both	6wks	N=138

Brockmann1	21309	organ weights	(none)	weight of liver [mg] Liver	QTL population	both	6wks	N=138

Brockmann1	21310	organ weights	(none)	weight of kidney [mg] Kidney	QTL population	both	6wks	N=138

Brockmann1	21311	organ weights	(none)	weight of spleen [mg] Spleen	QTL population	both	6wks	N=138

Brockmann1	21501	colony observation	(none)	number of offspring per litter: 5 classes [n] pupsize	QTL population	both	6wks	N=201

Brockmann1	21502	body weight	(none)	mouse body weight at week 6 [g] bw6	QTL population	both	6wks	N=201

Brockmann1	21503	organ weights	(none)	abdominal fat weight [mg] afw	QTL population	both	6wks	N=201

Brockmann1	21504	organ weights	(none)	abdominal fat percentage = %(Afw/body weight) [%] afp	QTL population	both	6wks	N=201

Brockmann1	21505	muscle weights	(none)	muscle weight [mg] mw	QTL population	both	6wks	N=201

Brockmann1	21506	organ weights	(none)	weight of liver [mg] liver	QTL population	both	6wks	N=201

Brockmann1	21507	organ weights	(none)	weight of kidney [mg] kidney	QTL population	both	6wks	N=201

Brockmann1	21508	organ weights	(none)	weight of spleen [mg] spleen	QTL population	both	6wks	N=201

Brockmann1	21509	hormone quantification	(none)	leptin level [ng/mL] leptin	QTL population	both	6wks	N=201

Brockmann1	21510	hormone quantification	(none)	insulin level [pmol/L] insulin	QTL population	both	6wks	N=201

Brockmann1	21511	hormone quantification	(none)	IGF-1 level [ng/mL] igf1	QTL population	both	6wks	N=201

Brockmann1	21512	hormone quantification	(none)	leptin level, log10 transformed [log(ng/mL)] lg_lept	QTL population	both	6wks	N=201

Brockmann1	21513	hormone quantification	(none)	insulin level, log10 transformed [log(pmol/L)] lg_ins	QTL population	both	6wks	N=201

Brockmann1	21514	hormone quantification	(none)	IGF1 level, log10 transformed [log(ng/mL)] lg_igf1	QTL population	both	6wks	N=201

Brockmann1	21515	lipid profile	(none)	triglyceride tg	QTL population	both	6wks	N=201

Brockmann1	21516	lipid profile	(none)	total cholesterol chol	QTL population	both	6wks	N=201

Brockmann1	21517	lipid profile	(none)	HDL cholesterol hdl	QTL population	both	6wks	N=201

Brockmann1	21518	lipid profile	(none)	triglycerides, log transformed lg_tg	QTL population	both	6wks	N=201

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Cross 1: DBA/2 x NMRI8 Brockmann1 a.k.a. Brockmann_2009a
Excel file: Data_Description_DBA2xNMRI8_Brockmann2009.xlsx
CSV file: Brockmann2009_DBA2xNMRI8_B37_Data.csv
Readme / text file: readme_Brockmann2009a.txt
QC plots, figures, etc.: QCReport_Brockmann2009a.pdf

Cross 2: DBA/2 x DU6i Brockmann1b a.k.a. Brockmann_2009b
Excel file: Data_Description_DBA2xDU6i_Brockmann2009.xlsx
CSV file: Brockmann2009_DBA2xDU6i_B37_Data.csv
Readme / text file: readme_Brockmann2009b.txt
QC plots, figures, etc.: QCReport_Brockmann2009b.pdf