MPD: Data set: Brinkmeyer1

Brinkmeyer1: Susceptibility to Theiler's murine encephalomyelitis virus in 5 Collaborative Cross strains of mice (2017)

Brinkmeyer-Langford CL, Rech R, Amstalden K, Kochan KJ, Hillhouse AE, Young C, Welsh CJ, Threadgill DW. Host genetic background influences diverse neurological responses to viral infection in mice. Sci Rep. 2017 Sep 22;7(1):12194. doi: 10.1038/s41598-017-12477-2. PubMed 28939838 FullText

Brinkmeyer1 downloads
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• Brinkmeyer1 supplementary data

Ontology terms mapped to Brinkmeyer1 measures:
MA:0000007 limb
MA:0000025 forelimb
MA:0000026 hindlimb
MA:0000168 brain
MP:0001259 abnormal body weight
MP:0001392 abnormal locomotor behavior
MP:0001406 abnormal gait
MP:0001440 abnormal grooming behavior
MP:0001516 abnormal motor coordination/balance
MP:0020185 altered susceptibility to viral infection
VT:0001259 body mass
VT:0001392 locomotor behavior trait
VT:0001440 grooming behavior trait
VT:0001516 motor coordination/balance trait
VT:0010439 response to viral infection trait
VT:0010731 gait trait

Participants	Candice L Brinkmeyer-Langford Texas A&M University, College Station, TX David W Threadgill Texas A&M University, College Station, TX With: Rech R, Amstalden K, Kochan KJ, Hillhouse AE, Young C, Welsh CJ
Contact	Candice L Brinkmeyer-Langford Brinkmeyer@tamu.edu Lab web site
Project type	Phenotype strain survey data set
MPD identifiers	Brinkmeyer1 MPD:644
Data changelog	No updates/corrections. Initial release date: 04/2019.
Formatted citation	Click above to copy-paste the entire citation for this MPD web page.

This study assessed the phenotypic consequences of TMEV infection in 5 CC strains of mice. Outcomes of long-term infection (3 mo) and acute vs. early chronic infection (7 vs. 28 d post infection) were evaluated using neurological and behavioral phenotyping tests along with body weight and clinical scores. Data for mock infected mice are in a supplemental data download file.

Summary of procedures that were conducted
• body weight
• health assessment	Clinical health assessment scores, TMEV infection.
• RNA expression profiling	TMEV transcript abundance.
• open field test	Locomotor activity, TMEV infection.
• gait analysis	DigiGait analysis and footprint analysis, TMEV infection.
• rotarod	Latency to fall from accelerating rotarod, TMEV infection.

Mice: CC 5 strains ♀♂ age 4wks 2 experimental groups

Sort/ Pick	Data set	Procedure / Protocol	Drug or Challenge	Phenotype Measure	Panel	No. of Strains	Sex	Age	Sample Size (Avg)
Brinkmeyer1:1	Brinkmeyer1	body weight	Theiler's murine encephalomyelitis virus	body weight, post infection [g] bw 64401 day 0 day 2 day 4 day 6 day 8 day 10 day 14 day 17 day 22 day 27 day 30 day 34 day 45 day 60 day 75 day 87	CC	5	both	4wks	N=5

Brinkmeyer1:17	Brinkmeyer1	health assessment	Theiler's murine encephalomyelitis virus	clinical score (0=normal, 6=moribund), post infection [score] clin_score 64417 day 0 day 2 day 4 day 6 day 8 day 12 day 18 day 24 day 29 day 34 day 42 day 46 day 68 day 75 day 82 day 87	CC	5	both	4wks	N=5

Brinkmeyer1:33	Brinkmeyer1	RNA expression profiling	Theiler's murine encephalomyelitis virus	TMEV transcript abundance (normalized), post infection TMEV 64433 day 7 day 28	CC	4	both	4wks	N=1

Brinkmeyer1:35	Brinkmeyer1	open field test	Theiler's murine encephalomyelitis virus	number of seconds spent grooming, 10 min test, post infection wk 3 (manual assessment) [s] grooming 64435	CC	5	both	4wks	N=2

Brinkmeyer1:36	Brinkmeyer1	open field test	Theiler's murine encephalomyelitis virus	number of times mouse reared, 10 min test, post infection wk 3 (manual assessment) [n] activity_rearing 64436	CC	5	both	4wks	N=2

Brinkmeyer1:37	Brinkmeyer1	open field test	Theiler's murine encephalomyelitis virus	horizontal movement latency, 30 min test, post infection wk 3 [s] horiz_latency 64437 trial 1 trial 2	CC	4	both	4wks	N=2

Brinkmeyer1:39	Brinkmeyer1	open field test	Theiler's murine encephalomyelitis virus	number of horizontal movements (beam breaks), 30 min test, post infection wk 3 [n] horiz_activity 64439 trial 1 trial 2	CC	4	both	4wks	N=2

Brinkmeyer1:41	Brinkmeyer1	open field test	Theiler's murine encephalomyelitis virus	horizontal distance traveled, 30 min test, post infection wk 3 [cm] horiz_distance 64441 trial 1 trial 2	CC	4	both	4wks	N=2

Brinkmeyer1:43	Brinkmeyer1	open field test	Theiler's murine encephalomyelitis virus	time spent moving horizontally, 30 min test, post infection wk 3 [s] horiz_time 64443 trial 1 trial 2	CC	4	both	4wks	N=2

Brinkmeyer1:45	Brinkmeyer1	open field test	Theiler's murine encephalomyelitis virus	horizontal movement velocity, 30 min test, post infection wk 3 [cm/s] horiz_velocity 64445 trial 1 trial 2	CC	4	both	4wks	N=2

Brinkmeyer1:47	Brinkmeyer1	open field test	Theiler's murine encephalomyelitis virus	number of vertical plane entries, 30 min test, post infection wk 3 [n] vert_activity 64447 trial 1 trial 2	CC	4	both	4wks	N=2

Brinkmeyer1:49	Brinkmeyer1	open field test	Theiler's murine encephalomyelitis virus	number of vertical plane episodes, 30 min test, post infection wk 3 [n] vert_episodes 64449 trial 1 trial 2	CC	4	both	4wks	N=2

Brinkmeyer1:51	Brinkmeyer1	open field test	Theiler's murine encephalomyelitis virus	time spent vertical, 30 min test, post infection wk 3 [s] vert_time 64451 trial 1 trial 2	CC	4	both	4wks	N=2

Brinkmeyer1:53	Brinkmeyer1	open field test	Theiler's murine encephalomyelitis virus	time spent resting, 30 min test, post infection wk 3 [s] resting 64453 trial 1 trial 2	CC	4	both	4wks	N=2

Brinkmeyer1:55	Brinkmeyer1	open field test	Theiler's murine encephalomyelitis virus	number of center entries, 30 min test, post infection wk 3 [n] center_entries 64455 trial 1 trial 2	CC	4	both	4wks	N=2

Brinkmeyer1:57	Brinkmeyer1	open field test	Theiler's murine encephalomyelitis virus	time spent in center, 30 min test, post infection wk 3 [s] center_time 64457 trial 1 trial 2	CC	4	both	4wks	N=2

Brinkmeyer1:59	Brinkmeyer1	open field test	Theiler's murine encephalomyelitis virus	time spent in periphery, 30 min test, post infection wk 3 [s] periphery_time 64459 trial 1 trial 2	CC	4	both	4wks	N=2

Brinkmeyer1:61	Brinkmeyer1	gait analysis	Theiler's murine encephalomyelitis virus	number of steps included in analysis as measured by DigiGait, post infection day 27, forelimb [n] num_steps_fore 64461 left right	CC	4	both	4wks	N=1

Brinkmeyer1:63	Brinkmeyer1	gait analysis	Theiler's murine encephalomyelitis virus	number of steps included in analysis as measured by DigiGait, post infection day 27, hindlimb [n] num_steps_hind 64463 left right	CC	4	both	4wks	N=1

Brinkmeyer1:65	Brinkmeyer1	gait analysis	Theiler's murine encephalomyelitis virus	duration of swing phase (no paw contact with belt) as measured by DigiGait, post infection day 27, forelimb [s] swing_dur_fore 64465 left right	CC	4	both	4wks	N=1