Project
protocol
-
Contents
Workflow
and
sampling
Step |
Procedure |
Equipment |
Data
collected |
1 |
Intrasession habituation |
Activity monitor |
Locomotor activity |
2 |
Intersession habituation
|
Activity monitor |
Locomotor activity |
Equipment
and
supplies
- Activity monitor: AccuScan VersaMax (Columbus OH)
- Sound attenuating chamber (Med Associates, St. Albans VT)
Procedure: Intrasession habituation of locomotor activity in a novel environment
- One mouse at a time is placed in the activity monitor and allowed to explore freely for 15 min.
- Locomotor activity is recorded in 5 min bins.
Procedure: Intersession habituation of locomotor activity in a novel environment
- One mouse at a time is placed in the activity monitor and allowed to explore freely for 15 min over two additional days (the first day's session is recorded in the above procedure).
- Locomotor activity is recorded.
Data
collected
by
investigator
- Intrasession habituation
- Distance traveled during each 5 min bin (15 min total)
- Computed habituation: Activity change score = distance traveled in the last 5 min/(distance during last 5 min + distance traveled in first 5 min)
- Intersession habituation
- Distance traveled during each 15 min session (over 3 days)
- Computed habituation: Activity change score = distance traveled on day 3/(distance on day 3 + distance on day 1)
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