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Berndt4 animal documentation

Aging study: Pulmonary adenomas in 28 inbred strains of mice, age 20 months   (2011)

Berndt A, Harrison DE, Paigen B, Sundberg JP
With: Cario CL, Silva KA, Kennedy VE

Berndt4 protocol

Acclimation & testing periods

Vendor	The Jackson Laboratory (JAX®, Bar Harbor, ME)
Ages	Mice obtained at 6-8 wks of age; tested at 20 months of age (+/- 28 days)
Feed	NIH31 5K52, LabDiet (PMI Nutritional International), given ad libitum
Water	Acidified, provided ad libitum
Temperature	Climate controlled, 21-23°C
Humidity	Climate controlled, humidity not specified
Housing	Duplex polycarbonate cages on pressurized, individually ventilated racks (Thoren Caging System) with a high-efficiency particulate air-filtered supply and exhaust; housed 4 per cage
Bedding	Not specified
Photoperiod	12h:12h light cycle
Health Status	Mouse colonies were regularly monitored (4 times/yr) for and found to be free of 17 viruses, 17 bacterial species, 2 Mycoplasma species, external and internal parasites, and the microsporidium E. cuniculi. See Berndt et al., 2011 for details.
Care and Use	Protocols approved by The Jackson Laboratory Animal Care and Use Committee, and conducted in compliance with the Niational Institutes of Health Guidelines for the Care and Use of Laboratory Animals.