MPD: Berndt3: animal documentation

Berndt3 animal documentation

Serum vitamin D levels in males of 18 inbred mouse strains (2011)

Berndt A
With: Savage HS, Stearns TM, Paigen B

Berndt3_Animal

Acclimation and testing periods

Supplier Male mice were obtained from The Jackson Laboratory (JAX®, Bar Harbor, ME)

Ages
Phenotypic analysis conducted at 11 wks of age

Feed
Mice were allowed ad libitum access to food pellets containing 6% fat (LabDiet 5K52, PMI Nutritional International, Bentwood, MO).

Water
Mice were allowed ad libitum access to acidified water (pH 2.8â3.2)

Housing
Mice of the same sex (maximum 5 per pen) were housed in duplex polycarbonate cages (31 X 31 X 214 cm) in pressurized individually ventilated mouse cages (Thoren Caging System, Hazelton, PA) with a high-efficiency particulate air-filtered supply and exhaust.

Bedding
Pine shavings

Photoperiod
12 h light/12 h dark cycle

Temperature
22 ± 1.0°C

Health status
The animal room at The Jackson Laboratory is SPF (specific pathogen free), regularly monitored for and free of viruses, bacterial species, Mycoplasma, and parasites, as described at JAX®mice. All animal protocols were reviewed and approved by the Animal Care and Use Committee at The Jackson Laboratory.

Supplier	Male mice were obtained from The Jackson Laboratory (JAX®, Bar Harbor, ME)
Ages	Phenotypic analysis conducted at 11 wks of age
Feed	Mice were allowed ad libitum access to food pellets containing 6% fat (LabDiet 5K52, PMI Nutritional International, Bentwood, MO).
Water	Mice were allowed ad libitum access to acidified water (pH 2.8â3.2)
Housing	Mice of the same sex (maximum 5 per pen) were housed in duplex polycarbonate cages (31 X 31 X 214 cm) in pressurized individually ventilated mouse cages (Thoren Caging System, Hazelton, PA) with a high-efficiency particulate air-filtered supply and exhaust.
Bedding	Pine shavings
Photoperiod	12 h light/12 h dark cycle
Temperature	22 ± 1.0°C
Health status	The animal room at The Jackson Laboratory is SPF (specific pathogen free), regularly monitored for and free of viruses, bacterial species, Mycoplasma, and parasites, as described at JAX®mice. All animal protocols were reviewed and approved by the Animal Care and Use Committee at The Jackson Laboratory.