Bennett1 project protocol

Body weight and growth curves in females of 97 ILSXISS recombinant inbred strains of mice   (2005)

Bennett B, Johnson TE
With: Carosone-Link PJ, Springett J, Hall J




Bennett1_Protocol

Project protocol — Contents
Workflow and sampling
Equipment
Reagents, supplies, and solutions
Procedure
Data
References



Workflow and sampling

Workflow

Step
Procedure
Age (days, ±)
Data Collected
1
Mice are weighed
31
2.9
body weight (bw)
2
Mice are weighed
61
2.2
3
Mice are weighed
91
2.6
4
Mice are weighed
122
3.0
5
Mice are weighed
151
4.1
6
Mice are weighed
179
4.1

Equipment

• Top loading balance

Reagents, supplies, solutions  

• Weighing tub
• Paper towel and disinfectant (95% Ethanol)

Procedure

a. Female mice from at least 1 litter are weighed.
b. Mice are weaned at 25 days of age and transferred to female-only or male-only cages (up to ~4 mice/cage).
c. Each mouse is weighed at target ages 30, 60, 90, 120, 150, and 180 days.
d. The weighing tub is cleaned after weighing each set of littermates.

Data collected by investigator

Body weight at target ages of 30, 60, 90, 120, 150, and 180-days


References

    Bennett B, Carosone-Link P, Zahniser NR, Johnson TE. Confirmation and fine mapping of ethanol sensitivity quantitative trait loci, and candidate gene testing in the LXS recombinant inbred mice. J Pharmacol Exp Ther. 2006 Oct;319(1):299-307. Epub 2006 Jun 27. PubMed 16803863

    Downing C, Carosone-Link P, Bennett B, Johnson T. QTL mapping for low-dose ethanol activation in the LXS recombinant inbred strains. Alcohol Clin Exp Res. 2006 Jul;30(7):1111-20. PubMed 16792557