Acclimation and testing periods
Supplier All breeding and testing of live mice* were conducted in the Specific Pathogen Free (SPF) facility at the Institute for Behavioral Genetics, University of Colorado at Boulder. Ages 30-180 days Feed Sterilized rodent diet by Teklad (4% fat, 24% protein) given ad libitum Water Given ad libitum Housing Mice were weaned and transferred to male-only or female-only cages Bedding Harlan Softcob bedding Photoperiod 12:12 hr L:D cycle Humidity 12% relative Temperature 22.8 °C (73°F) Regulatory compliance All procedures followed guidelines developed by the National Institutes of Health (NIH) and the Institutional Animal Care and Use Committee (IACUC).
*The ILSXISS#/Tej (formerly LXS) recombinant inbred lines were generated from crosses between ILS/Ibg (Inbred Long Sleep) and ISS/Ibg (Inbred Short Sleep). These two progenitor strains were developed from the LS and SS selected lines developed from a heterogenous stock derived from an 8 way cross (A, AKR, BALB/c, C3H/2, C57BL, DBA/2, IS/Bi and RIII). ILS and ISS are the product of inbreeding LS and SS, lines selected for long or short duration of loss-of-righting response following high-dose intraperitoneal administration of ethanol. To produce RI strains, ILS and ISS mice were intercrossed and 4 reciprocal matings of F1 mice were used to produce the F2 generation. Each of the four F2 crosses generated a unique combination of Y Chromosome and maternal (mitochondrial) contributions. Breedings were conducted in the Specific Pathogen Free (SPF) facility at the Institute for Behavioral Genetics, University of Colorado at Boulder (see Bennett et. al., 2005).
Note: Strain names for mice tested prior to importation to The Jackson Laboratory have been updated using current strain nomenclature and assigned JAX vendor codes (if available) in order to facilitate comparisons across projects.