Auwerx1: Diet effects on metabolic phenotypes in males of 55 BXD recombinant inbred strains of mice fed a high-fat diet (2016)

Williams EG, Wu Y, Jha P, Dubuis S, Blattmann P, Argmann CA, Houten SM, Amariuta T, Wolski W, Zamboni N, Aebersold R, Auwerx J. Systems proteomics of liver mitochondria function. Science. 2016 Jun 10;352(6291):aad0189. doi: 10.1126/science.aad0189.   PubMed 27284200  

Jha P, McDevitt MT, Halilbasic E, Williams EG, Quiros PM, Gariani K, Sleiman MB, Gupta R, Ulbrich A, Jochem A, Coon JJ, Trauner M, Pagliarini DJ, Auwerx J. Genetic Regulation of Plasma Lipid Species and Their Association with Metabolic Phenotypes. Cell Syst. 2018 Jun 27;6(6):709-721.e6. doi: 10.1016/j.cels.2018.05.009. Epub 2018 Jun 13.   PubMed 29909275     FullText

Auwerx1 downloads
• Download Auwerx1 project data set     animal data, as uploaded
• Download Auwerx1 animal data matrix     with factor-related expansion applied as necessary
• Download Auwerx1 strain means, SD, N, etc.     one row per strain/sex/measure
Ontology terms mapped to Auwerx1 measures:
    MA:0000009   adipose tissue
    MA:0000010   cardiovascular system
    MA:0000059   blood
    MA:0000072   heart
    MA:0000327   respiratory system
    MA:0000358   liver
    MA:0000368   kidney
    MA:0000473   subcutaneous adipose tissue
    MA:0002424   soleus
    MA:0002550   epididymal fat pad
    MA:0002975   perirenal fat
    MA:0003148   skeletal muscle
    MP:0000180   abnormal circulating cholesterol level
    MP:0000181   abnormal circulating LDL cholesterol level
    MP:0000184   abnormal circulating HDL cholesterol level
    MP:0000187   abnormal triglyceride level
    MP:0000188   abnormal circulating glucose level
    MP:0000202   abnormal circulating alkaline phosphatase level
    MP:0000203   abnormal circulating aspartate transaminase level
    MP:0001259   abnormal body weight
    MP:0001392   abnormal locomotor behavior
    MP:0001553   abnormal circulating free fatty acids level
    MP:0001560   abnormal circulating insulin level
    MP:0001573   abnormal circulating alanine transaminase level
    MP:0001586   abnormal erythrocyte cell number
    MP:0001629   abnormal heart rate
    MP:0001781   abnormal white adipose tissue amount
    MP:0002332   abnormal exercise endurance
    MP:0002336   abnormal pulmonary gas exchange
    MP:0002596   abnormal hematocrit
    MP:0002707   abnormal kidney weight
    MP:0002943   abnormal lactate dehydrogenase level
    MP:0003959   abnormal lean body mass
    MP:0004152   abnormal circulating iron level
    MP:0004774   abnormal bile salt level
    MP:0004847   abnormal liver weight
    MP:0004857   abnormal heart weight
    MP:0005288   abnormal oxygen consumption
    MP:0005291   abnormal glucose tolerance
    MP:0005328   abnormal circulating creatinine level
    MP:0005449   abnormal food intake
    MP:0005452   abnormal adipose tissue amount
    MP:0005535   abnormal body temperature
    MP:0008551   abnormal circulating interferon-gamma level
    MP:0008552   abnormal circulating tumor necrosis factor level
    MP:0008590   abnormal circulating interleukin-10 level
    MP:0008595   abnormal circulating interleukin-6 level
    MP:0008610   abnormal circulating interleukin-15 level
    MP:0008634   abnormal circulating interleukin-18 level
    MP:0008804   abnormal circulating amylase level
    MP:0010209   abnormal circulating chemokine level
    MP:0010237   abnormal skeletal muscle weight
    MP:0010695   abnormal blood pressure regulation
    MP:0011888   abnormal circulating total protein level
    MP:0013403   abnormal circulating lactate level
    MP:0020165   abnormal soleus weight
    MP:0020279   abnormal creatine kinase level
    VT:0000180   blood cholesterol amount
    VT:0000181   blood LDL cholesterol amount
    VT:0000183   blood pressure trait
    VT:0000184   blood HDL cholesterol amount
    VT:0000187   triglyceride amount
    VT:0000188   blood glucose amount
    VT:0001259   body mass
    VT:0001392   locomotor behavior trait
    VT:0001431   eating behavior trait
    VT:0001553   blood free fatty acid amount
    VT:0001560   blood insulin amount
    VT:0001586   erythrocyte quantity
    VT:0001777   body temperature regulation trait
    VT:0002121   metabolism trait
    VT:0002332   exercise endurance trait
    VT:0002336   pulmonary gas exchange trait
    VT:0002338   pulmonary ventilation trait
    VT:0002707   kidney mass
    VT:0003081   soleus morphology trait
    VT:0003402   liver mass
    VT:0004152   blood iron amount
    VT:0005328   blood creatinine amount
    VT:0005452   adipose amount
    VT:0005567   blood total protein amount
    VT:0007028   heart mass
    VT:0008559   interferon-gamma secretion trait
    VT:0008590   blood interleukin-10 amount
    VT:0008595   blood interleukin-6 amount
    VT:0008610   blood interleukin-15 amount
    VT:0008634   blood interleukin-18 amount
    VT:0008721   chemokine amount
    VT:0010421   epididymal fat pad mass
    VT:0010429   renal fat pad mass
    VT:0010471   exploratory behavior trait
    VT:0010477   blood lactate dehydrogenase amount
    VT:0010482   body fat mass
    VT:0010483   body lean mass
    VT:0010616   blood lactate amount
    VT:0015088   glucose metabolism trait
    VT:1000047   blood creatine kinase amount
    VT:1000472   subcutaneous adipose mass
    VT:1000712   skeletal muscle mass
    VT:2000009   heart pumping trait
Investigators Evan G Williams       Ecole polytechnique fédérale de Lausanne,  Lausanne SWITZERLAND
Yibo Wu       Institute of Molecular Systems Biology,  Zurich SWITZERLAND
Ruedi Aebersold       Institute of Molecular Systems Biology,  Zurich SWITZERLAND
Johan Auwerx       Ecole polytechnique fédérale de Lausanne,  Lausanne SWITZERLAND
Participants Bou Sleiman M, Rapin A, Jha P, Dubuis S, Blattmann P, Argmann CA, Houten SM, Amariuta T, Wolski W, Zamboni N
ContactJohan Auwerx     Lab web site
Funding Provided ByNIH AG043930; EPFL, ETHZ, ERC (AdG-670821 and Proteomics v3.0; AdG-233226), the AgingX program of the Swiss Initiative for Systems Biology (51RTP0-151019, 2013/134, and 2013/153), SNSF (31003A-140780, 31003A-143914, and CSRII3-13620
Project type Phenotype strain survey data set
MPD identifiersAuwerx1     MPD:1023
Data changelog No updates/corrections.       Initial release date: 11/2020.
Formatted citation
Click above to copy-paste the entire citation for this MPD web page.
BXD male mice were fed either a regular chow diet (CD) or a high‑fat diet (HFD, 60% kCal fat, 20% kCal protein and 20% kCal of carbohydrates) from 8 to 29 weeks of age. Various physiological and metabolic traits were measured throughout the course of the study, as well as organs weighed such as heart, kidney, liver, skeletal tissue, and fat pads. The effects of HFD diet were measured on body weight, cardiovascular function, respiratory function, activity, blood glucose and insulin levels, body temperature upon exposure to cold, red blood cell count and hematocrit, immune cell signaling molecule detection, circulating lipids, and other metabolic traits.

Experimental groups in this study:
• Chow diet (CD)     • High fat diet (HFD)    

Procedures conducted:
• body weight  8-29 wks of age.
• NMR  Body composition.
• intake monitoring  Food intake.
• monitoring system  Oxygen consumption and respiratory exchange ratio (RER); activity.
• glucose tolerance  Glucose post oral gavage: 0, 15, 30, 45, 60, 90, 120, 150, 180 min plus area under curve.
• hormone quantification  Glucose post oral gavage: 0, 15, 30 min plus area under curve.
• tail cuff  Blood pressure and heart rate.
• body temperature  In cold room: 0, 1, 2, 3, 4, 5, 6 h.
• treadmill endurance test  Distance traveled.
• running wheel monitoring  Distance traveled, speed.
• metabolic panel  Enzymes, bile acid, proteins, creatinine, glucose, iron, lactate.
• lipid profile  Cholesterol, HDL, LDL, free fatty acids, triglyceride.
• cell signaling molecule detection  INF-gamma, interleukins, monocyte chemoattractant, macrophage inflammatory protein, T cell RANTES, TNF-alpha.
• complete blood count  RBC, hematocrit.
• organ weights  Skeletal muscle, soleus muscle, heart, kidney, liver, various fat pads.

Mice: BXD w/par   57 strains   ♂   age 8-29 wks   2 experimental groups