To identify genes and pathways that contribute to obesity-induced type II diabetes,
we constructed an F2 intercross between the diabetes-resistant C57BL/6J and diabetes-susceptible BTBR T+ tf/J mouse strains.
All F2s were genetically obese through introgression of the leptin mutation (Lep ob/ob) and all were sacrificed at 10 weeks of age.
We conducted genome-wide gene expression profiling in six tissues: adipose, gastrocnemius muscle, hypothalamus, pancreatic islets, kidney, and liver.
Gene expression was assayed with custom Agilent two-color ink-jet microarrays. Mice were genotyped with the Affymetrix 5K GeneChip.
1) Identification of Slco1a6 as a candidate gene that broadly affects gene expression in mouse pancreatic islets.
Tian J, Keller MP, Oler AT, Rabaglia ME, Schueler KL, Stapleton DS, Broman AT, Zhao W, Kendziorski CM, Yandell BS, Hagenbuch B, Broman KW, Attie AD (2015) bioRxiv, doi: 10.1101/020974
2) Identification and correction of sample mix-ups in expression genetic data: A case study.
Broman KW, Keller MP, Broman AT, Kendziorski C, Yandell BS, Sen A, Attie AD (2014) arXiv:1402.2633
Each of the .zip files is approx 950 MB download size.
The csv files posted below supersede those found in the *_clean.zip archive.