ITP phenotypes:   rotarod     nordihydroguaiaretic acid

Trial code: NDGA_hi Dose: 5000 ppm   initiated at age: 6 mo Sex: f Cohort: C2010
Trial code: NDGA_mid Dose: 2500 ppm   initiated at age: 6 mo Sex: m Cohort: C2010
The plots below show the means with ± SEM error bars





ANOVA

Factor Df Sum Sq Mean Sq F value Pr(>F)
group 2 982481.7978 491240.8989 339.4694 < 0.0001
time 4 1023435.1293 255858.7823 176.8099 < 0.0001
group:time 8 38703.8167 4837.9771 3.3433 0.0008
Residuals 2013 2912980.8254 1447.0844 NA NA


Means

group time mean SE
Old_Control time_d1 13.6331 1.2161
Old_Control time_d2 28.1649 2.0158
Old_Control time_d3 39.05 2.3938
Old_Control time_d4 56.8806 3.2546
Old_Control time_d5 64.2368 3.1919
Old_NDGA_hi time_d1 27.2073 2.1252
Old_NDGA_hi time_d2 53.425 3.0897
Old_NDGA_hi time_d3 74.4022 3.7884
Old_NDGA_hi time_d4 84.2642 4.4475
Old_NDGA_hi time_d5 89.15 4.333
Young_Control time_d1 49.9521 2.7044
Young_Control time_d2 77.9925 3.7289
Young_Control time_d3 103.5358 3.9772
Young_Control time_d4 116.41 4.2094
Young_Control time_d5 124.2983 3.886


Pairwise comparisons (Tukey multiple-test correction)

contrast time estimate SE df t.ratio p.value
Old_Control - Old_NDGA_hi time_d1 -13.5742 4.4074 2013 -3.0799 0.0059
Old_Control - Young_Control time_d1 -36.319 4.7059 2013 -7.7177 < 0.0001
Old_NDGA_hi - Young_Control time_d1 -22.7448 4.6921 2013 -4.8475 < 0.0001
Old_Control - Old_NDGA_hi time_d2 -25.2601 4.4971 2013 -5.617 < 0.0001
Old_Control - Young_Control time_d2 -49.8276 4.6521 2013 -10.7107 < 0.0001
Old_NDGA_hi - Young_Control time_d2 -24.5675 4.7644 2013 -5.1565 < 0.0001
Old_Control - Old_NDGA_hi time_d3 -35.3522 4.4901 2013 -7.8734 < 0.0001
Old_Control - Young_Control time_d3 -64.4858 4.6454 2013 -13.8818 < 0.0001
Old_NDGA_hi - Young_Control time_d3 -29.1336 4.7644 2013 -6.1149 < 0.0001
Old_Control - Old_NDGA_hi time_d4 -27.3836 4.566 2013 -5.9973 < 0.0001
Old_Control - Young_Control time_d4 -59.5294 4.7019 2013 -12.6606 < 0.0001
Old_NDGA_hi - Young_Control time_d4 -32.1458 4.781 2013 -6.7236 < 0.0001
Old_Control - Old_NDGA_hi time_d5 -24.9132 4.5486 2013 -5.4771 < 0.0001
Old_Control - Young_Control time_d5 -60.0615 4.7019 2013 -12.7738 < 0.0001
Old_NDGA_hi - Young_Control time_d5 -35.1483 4.7644 2013 -7.3773 < 0.0001










ANOVA

Factor Df Sum Sq Mean Sq F value Pr(>F)
group 2 437649.7339 218824.8669 163.5492 < 0.0001
time 4 744291.7042 186072.9261 139.0705 < 0.0001
group:time 8 16937.6674 2117.2084 1.5824 0.1251
Residuals 1648 2204984.4011 1337.976 NA NA


Means

group time mean SE
Old_Control time_d1 21.9887 1.754
Old_Control time_d2 36.7472 2.3668
Old_Control time_d3 58.1647 3.095
Old_Control time_d4 79.9375 3.838
Old_Control time_d5 80.1235 4.2448
Old_NDGA_mid time_d1 51.725 3.5356
Old_NDGA_mid time_d2 73.8889 4.3171
Old_NDGA_mid time_d3 84.3889 4.0367
Old_NDGA_mid time_d4 103.4806 4.7403
Old_NDGA_mid time_d5 105.8278 4.4437
Young_Control time_d1 59.02 2.6027
Young_Control time_d2 74.4275 3.0001
Young_Control time_d3 95.7383 3.6512
Young_Control time_d4 113.7975 3.6463
Young_Control time_d5 102.0867 3.5408


Pairwise comparisons (Tukey multiple-test correction)

contrast time estimate SE df t.ratio p.value
Old_Control - Old_NDGA_mid time_d1 -29.7363 5.2387 1648 -5.6763 < 0.0001
Old_Control - Young_Control time_d1 -37.0313 4.4733 1648 -8.2783 < 0.0001
Old_NDGA_mid - Young_Control time_d1 -7.295 5.4528 1648 -1.3378 0.3744
Old_Control - Old_NDGA_mid time_d2 -37.1417 5.2796 1648 -7.0349 < 0.0001
Old_Control - Young_Control time_d2 -37.6803 4.5212 1648 -8.3341 < 0.0001
Old_NDGA_mid - Young_Control time_d2 -0.5386 5.4528 1648 -0.0988 0.9946
Old_Control - Old_NDGA_mid time_d3 -26.2242 5.3311 1648 -4.9191 < 0.0001
Old_Control - Young_Control time_d3 -37.5736 4.5813 1648 -8.2016 < 0.0001
Old_NDGA_mid - Young_Control time_d3 -11.3494 5.4528 1648 -2.0814 0.094
Old_Control - Old_NDGA_mid time_d4 -23.5431 5.3311 1648 -4.4161 < 0.0001
Old_Control - Young_Control time_d4 -33.86 4.5813 1648 -7.391 < 0.0001
Old_NDGA_mid - Young_Control time_d4 -10.3169 5.4528 1648 -1.8921 0.1413
Old_Control - Old_NDGA_mid time_d5 -25.7042 5.3311 1648 -4.8215 < 0.0001
Old_Control - Young_Control time_d5 -21.9631 4.5813 1648 -4.7941 < 0.0001
Old_NDGA_mid - Young_Control time_d5 3.7411 5.4528 1648 0.6861 0.7716










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