Berndt2: Unrestrained plethysmograph and anesthetized forced oscillation methods of measuring lung function in 29 inbred strains of mice (2010)

Berndt A, Leme AS, Williams LK, Von Smith R, Savage HS, Stearns TM, Tsaih SW, Shapiro SD, Peters LL, Paigen B, Svenson KL. Comparison of unrestrained plethysmography and forced oscillation for identifying genetic variability of airway responsiveness in inbred mice. Physiol Genomics. 2011 Jan 7;43(1):1-1Epub 2010 Sep 7.   PubMed 20823217     FullText


       
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Participants Annerose Berndt       University of Pittsburgh School of Medicine Pittsburgh, PA
Adriana S Leme       University of Pittsburgh School of Medicine Pittsburgh, PA
Beverly Paigen       The Jackson Laboratory Bar Harbor, ME
Steven D Shapiro       University of Pittsburgh School of Medicine Pittsburgh, PA
Karen L Svenson       The Jackson Laboratory Bar Harbor, ME
ContactAnnerose Berndt     anb128@pitt.edu     Lab web site
AcknowledgementsFunding provided by HL66611, HL83069, CA34196
Project type Phenotype strain survey data set
MPD identifiersBerndt2     MPD:351
Data changelog 1 update/correction.       Initial release date: 03/2011.
Formatted citation
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Mice were assessed for respiratory parameters at baseline and then again after methacholine (MCh) challenge using two different techniques in two cohorts: 1) unrestrained plethysmograph technique (BUXCO), given MCh doses of 5, 10, and 20 mg/mL; 2) anesthetized forced oscillation technique (FlexiVent), given MCh doses of 1, 3, 10, and 30 mg/mL . The "MCh response index" is the average slope of the MCh challenge series (see protocol for details).

Summary of procedures that were conducted
• plethysmography Lung, respiratory function. Baseline vs. methacholine-challenged (several doses).
• ventilator Lung, respiratory function. Anesthetized forced oscillation. Baseline vs. methacholine-challenged (several doses).
Mice: inbred   29 strains   ♀♂   age 9-12wks   2 cohorts

Select table page: First Prev Next Last
Data set MPD ID Procedure / Protocol Drug or Challenge Phenotype Measure Panel No. of Strains Sex Age Sample Size (Avg)
    Berndt2 35101 plethysmography methacholine minute ventilation   [mL/min]     saline    5 MCh    10 MCh    20 MCh    response index inbred 29 both 9-12wks N=8
    Berndt2 35108 plethysmography methacholine breath frequency   [n/min]     saline    5 MCh    10 MCh    20 MCh    response index inbred 29 both 9-12wks N=8
    Berndt2 35114 plethysmography methacholine inspiratory phase   [s]     saline    5 MCh    10 MCh    20 MCh    response index inbred 29 both 9-12wks N=8
    Berndt2 35121 plethysmography methacholine expiratory phase   [s]     saline    5 MCh    10 MCh    20 MCh    response index inbred 29 both 9-12wks N=8
    Berndt2 35128 plethysmography methacholine respiratory cycle   [s]     saline    5 MCh    10 MCh    20 MCh    response index inbred 29 both 9-12wks N=8
    Berndt2 35134 plethysmography methacholine duty cycle   [ratio]     saline    5 MCh    10 MCh    20 MCh    response index inbred 29 both 9-12wks N=8
    Berndt2 35141 plethysmography methacholine inspiratory flow rate   [mL/kg/s]     saline    5 MCh    10 MCh    20 MCh    response index inbred 29 both 9-12wks N=8
    Berndt2 35148 plethysmography methacholine expiratory flow rate   [mL/kg/s]     saline    5 MCh    10 MCh    20 MCh    response index inbred 29 both 9-12wks N=8
    Berndt2 35154 plethysmography methacholine respiratory flow rate   [mL/kg/s]     saline    5 MCh    10 MCh    20 MCh    response index inbred 29 both 9-12wks N=8
    Berndt2 35161 plethysmography methacholine enhanced pause     saline    5 MCh    10 MCh    20 MCh    response index inbred 29 both 9-12wks N=8
    Berndt2 35168 plethysmography methacholine tidal volume   [mL]     saline    5 MCh    10 MCh    20 MCh    response index inbred 29 both 9-12wks N=8
    Berndt2 35181 ventilator methacholine airway resistance (H20 pressure)   [cm/mL/s]     saline    1 MCh    3 MCh    10 MCh    30 MCh    response index inbred 29 both 9-12wks N=9
    Berndt2 35191 ventilator methacholine lung tissue dynamic elastance (H20 pressure)   [cm/mL]     saline    1 MCh    3 MCh    10 MCh    30 MCh    response index inbred 29 both 9-12wks N=9
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