Schughart4: Susceptibility to influenza A (H3N2) viral infection: body weight, viral loads and leukocyte profiles in females of the 8 inbred founder strains of the Collaborative Cross (2016)

Leist SR, Pilzner C, van den Brand JM, Dengler L, Geffers R, Kuiken T, Balling R, Kollmus H, Schughart K. Influenza H3N2 infection of the collaborative cross founder strains reveals highly divergent host responses and identifies a unique phenotype in CAST/EiJ mice. BMC Genomics. 2016 Feb 27;17:143. doi: 10.1186/s12864-016-2483-y.   PubMed 26921172     FullText


       
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Participants Sarah R Leist       Helmholtz Center for Infection Research Braunschweig GERMANY
Heike Kollmus       Helmholtz Center for Infection Research Braunschweig GERMANY
Klaus Schughart       Helmholtz Center for Infection Research Braunschweig GERMANY
ContactKlaus Schughart     Klaus.Schughart@helmholtz-hzi.de     Lab web site
Part of a series: • study 1   see Schughart3
• study 2   (this project) Schughart4
• study 3   see Schughart5
Project type Phenotype strain survey data set
MPD identifiersSchughart4     MPD:587
Data changelog No updates/corrections.       Initial release date: 01/2017.
Formatted citation
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Mice infected with influenza A H3N2 virus (one dose) were analyzed for daily weight loss for up to 30 days. The mouse-adapted virus strain influenza A/HK/01/68 was used. Viral loads and leukocyte profiles are also available. Overall survival and mortality rates are available from females and males in Schughart3, and lung weights are available from females in Schughart5.

Summary of procedures that were conducted
• body weight Influenza A (H3N2) virus vs. controls. Several timepoints.
• complete blood count White blood cell count and differentials. Influenza A (H3N2) virus vs. controls.
• in vitro assay Viral load in lungs after infection with influenza A (H3N2) virus.
Mice: inbred   8 strains   ♀   age 8-12wks   2 cohorts

Select table page: First Prev Next Last
Data set MPD ID Procedure / Protocol Drug or Challenge Phenotype Measure Panel No. of Strains Sex Age Sample Size (Avg)
    Schughart4 58701 body weight (none) body weight pre-infection   [g]     control group    treated group inbred w/CC8 8 f 8-12wks N=13
    Schughart4 58711 body weight (none) percent change in body weight, control group   [%]     days post-treatment (saline):    1    2    3 inbred w/CC8 8 f 8-12wks N=6
    Schughart4 58721 body weight influenza A (H3N2) virus percent change in body weight, treated group   [%]     days post-infection:    1    2    3    4    5    6    7    8    9    10    11    12    13    14    18    30 inbred w/CC8 8 f 8-12wks N=11
    Schughart4 58741 complete blood count influenza A (H3N2) virus  control white blood cell count (WBC; per volume x 103), control group, day 3   [n/µL]  WBC_ctrl_d3 inbred w/CC8 8 f 8-12wks N=5
    Schughart4 58743 complete blood count influenza A (H3N2) virus white blood cell count (WBC), treated group   [n/µL]     days post-infection:    3    5    8    18    30 inbred w/CC8 8 f 8-12wks N=6
    Schughart4 58751 complete blood count influenza A (H3N2) virus  control lymphocyte differential (percent of total WBC), control group, day 3   [%]  pctLYM_ctrl_d3 inbred w/CC8 8 f 8-12wks N=5
    Schughart4 58753 complete blood count influenza A (H3N2) virus lymphocyte differential, treated group   [%]     days post-infection:    3    5    8    18    30 inbred w/CC8 8 f 8-12wks N=6
    Schughart4 58761 complete blood count influenza A (H3N2) virus  control monocyte differential (percent of total WBC), control group, day 3   [%]  pctMONO_ctrl_d3 inbred w/CC8 8 f 8-12wks N=5
    Schughart4 58763 complete blood count influenza A (H3N2) virus monocyte differential, treated group   [%]     days post-infection:    3    5    8    18    30 inbred w/CC8 8 f 8-12wks N=6
    Schughart4 58771 complete blood count influenza A (H3N2) virus  control granulocyte differential (percent of total WBC), control group, day 3   [%]  pctGRA_ctrl_d3 inbred w/CC8 8 f 8-12wks N=5
    Schughart4 58773 complete blood count influenza A (H3N2) virus granulocyte differential, treated group   [%]     days post-infection:    3    5    8    18    30 inbred w/CC8 8 f 8-12wks N=6
    Schughart4 58783 in vitro assay influenza A (H3N2) virus viral load in lungs (titer, FFU per lung) log10 (x+1), treated group   [log(n)]     days post-infection:    3    5    8 inbred w/CC8 8 f 8-12wks N=5
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