Neoplasia induction in NOD.CB17-Prkdcscid/J by passage of live tumor cells (HT-29)
Animal Documentation
Shipment: Mice were obtained at 6 wks of age from The Jackson Laboratory (Bar Harbor, ME)
Historical anchor: Date of Birth = 24 APR 2007 ± 3 days
Acclimation and testing periods:
Test facility | JaxWest, Sacramento CA USA |
Tagging | Ear-notch upon arrival |
Acclimation duration at test facility | 12 days |
Age at testing | 7-12 wks |
Feed | LabDiet 5LL4, ad libitum |
Water | Filtered tap (Sacramento CA), acidified to pH 2.8-3.2 |
Housing | Positively ventilated polycarbonate cages with HEPA filtered air; 4 mice per cage |
Bedding | Bed-o-cob, changed every two weeks or as needed |
Ventilation | 15 air exchanges per hour |
Lighting | Artificial, fluorescent |
Photoperiod | 12h-light/dark cycle (0700h to 1900h) |
Temperature | 22° ± 4°C |
Relative humidity | 50% ± 15% |
Health status | Clinical observations conducted regularly |