Jaxwest2 project protocol
Neoplasia induction in NOD.CB17-Prkdcscid/J female mice by passage of live tumor cells (HT29) (2007)
Jackson Laboratory
With: Yang Y, Rodriguez R, Braun M, Clary D
Neoplasia induction in NOD.CB17-Prkdcscid/J by passage of live tumor cells (HT-29)
Protocol
Purpose: To provide normative data for testing biologically-active compounds in the immunodeficient strain, NOD.CB17-Prkdcscid/J, which is an excellent host for xenografts. The human colorectal adenocarcinoma cell line, HT-29, is used for tumor induction.
This protocol indicates a treatment schedule. Data from these tests are proprietary; however, data are available from controls and pre-treatment metrics for mice in the Test Group.
By posting this data, JAX® In Vivo Services contributes to community research resources and fulfills an agreement with clients to make normative data public.
Day |
Event
|
Intervention |
Body
Weight |
Tumor
Metrics |
Experimental
Groups
|
|||
Control no intervention |
Control
vehicle
only |
Test
Group |
Age
(wks) |
|||||
1 |
Arrive
at
test
facility |
Y |
Y |
Y |
Y |
6 |
||
12 |
Y |
Y |
Y |
Y |
7.5 |
|||
Inject
tumor
cells
(HT29) |
Y |
Y |
Y |
|||||
15 |
Y |
Y |
Y |
Y |
8 |
|||
17 |
Y |
Y |
Y |
Y |
Y |
|||
18 |
Y |
Y |
Y |
Y |
||||
19 |
Y |
Y |
Y |
Y |
Y |
|||
Treatment
1 |
||||||||
22 |
Y |
Y |
Y |
Y |
9 |
|||
24 |
Y |
Y |
Y |
Y |
||||
26 |
Y |
Y |
Y |
Y |
||||
Treatment
2 |
||||||||
29 |
Y |
Y |
Y |
Y |
10
|
|||
31 |
Y |
Y |
Y |
Y |
||||
33 |
Y |
Y |
Y |
Y |
||||
Treatment
3 |
||||||||
36 |
Y |
Y |
Y |
Y |
11 |
|||
38 |
Y |
Y |
Y |
Y |
||||
40 |
Y |
Y |
Y |
Y |
12 |
|||
Treatment
4 |
||||||||
43 |
Y |
Y |
Y |
|||||
44 |
Necropsy |
Y |
Y
|
Y |
Y |
|||
DATA
AVAILABLE
|
Y |
Y
|
Y |
Y |
||||
Experimental Groups:
Control (no vehicle) mice receive the HT29 injection only and no further treatment.
Control-vehicle: mice receive the HT29 injection followed by weekly injections of saline (vehicle; intra-peritoneal).
Test Group (treatment): Proprietary
Saline (sterile)
HT29 tumor cell line (ATCC #HTB-38) suspension in saline
Mice receive a single injection of up to 1x106 cells (HT29) in 100 µL, subcutaneously in the dorsal flank.
One week later, Control-Vehicle mice begin receiving weekly injections of saline, for a total of four injections.
Mice are weighed and tumor sites are palpated three times a week (measured with a digital caliper). Body weights and tumor metrics are recorded.
Tumors are harvested at the end of the study and weighed, snap frozen, or fixed in formalin.
Tumor volume = (tumor length * tumor width2) ÷ 2
Body weights and tumor metrics were measured several times a week over the course of the experiment (see Workflow). Changes in body weights and tumor volume were determined as a percentage of prior measurements (specified in data descriptions). Per-animal data are available along with the latency (days) for tumor volume to reach 500 and 1500 mm3.
Data Summary Graph for Tumor Volume
