Mogil3 project protocol

Spared nerve injury induced mechanical allodynia in 18 inbred strains of mice   (2012)

Mogil JS, Salter MW
With: Sorge RE, Trang T

See also: Mogil3 animal documentation


Mogil3_protocol

Project protocol - Contents

Workflow and sampling

Step
Procedure performed
Data collected
1
von Frey testing
baseline
2
Spared nerve injury procedure
-
3
von Frey testing on post-operative days (1, 4, 7, 14, 21, 28)
% allodynia

Equipment and supplies

  • Surgical equipment
  • von Frey testing cubicles (see details below)
  • Nylon monofilaments (Stoelting Touch Test Sensory Evaluator Kit)

Reagents and solutions

  • Isoflurane and oxygen

Acclimation to test conditions

Mice habituated to the vivarium for at least one week before testing,

Procedure: von Frey testing

    1. Mice are placed individually in transparent Plexiglas cubicles (5 cm wide x 8.5 cm long x 6 cm high, where each cubical is separated from another by an opaque divider) placed on a perforated metal floor (with 5 mm diameter holes placed 7 mm apart).
    2. Mice are habituated at least 2 h before testing.
    3. Nylon monofilaments (corresponding to 0.015 to 1.3 g bending force; calibrated weekly) are firmly applied to the plantar surface of the hindpaw (alternating the side of the body being tested), until they bowed for 5 s.
    4. Mice are tested only when alert or resting, not while grooming.
    5. Three separate threshold determinations are made and averaged.

Procedure: Spared nerve injury (SNI) and hypersensitivity testing

    1. Mice are anesthetized with isoflurane and oxygen.
    2. Mice receive unilateral SNI according to published procedures (Shields et al., 2003).
    3. Von Frey fibers are applied to the lateral aspect of the plantar hindpaw (as detailed above) on post-operative days 1, 4, 7, 14, 21, and 28.

Definitions and calculations

  • SNI: spared nerve injury

Data collected by investigator

  • baseline hypersensitivity
  • 50% allodynia


References

    Shields SD, Eckert WA 3rd, Basbaum AI. Spared nerve injury model of neuropathic pain in the mouse: a behavioral and anatomic analysis. J Pain. 2003 Oct;4(8):465-70. PubMed 14622667