Reciprocal
mating
between
parental
inbred
strain
129S
(red
arrow/allele),
B6
(black),
and
C3
(blue)
is
illustrated,
wherein
both
male
and
female
from
one
inbred
strain
are
mated
to
another
inbred
strain.
Eight
inbred
strains
depicted
above
are
bred
reciprocally
to
each
other
to
generate
the
following
F1
offspring:
129SB6F1,
129SB8F1,
129SC3F1,
129SCF1,
129SNZBF1,
129SPWF1,
129SSJLF1,
B6129SF1,
B6B8F1,
B6C3F1,
B6CF1,
B6NZBF1,
B6PWF1,
B6SJLF1,
C3129SF1,
C3B6F1,
C3B8F1,
C3CF1,
C3NZBF1,
C3PWF1,
C3SJLF1,
B8129SF1,
B8B6F1,
B8C3F1,
B8CF1,
B8NZBF1,
B8PWF1,
B8SJLF1,
C129SF1,
CB6F1,
CB8F1,
CC3F1,
CNZBF1,
CSJLF1,
NZB129SF1,
NZBB6F1,
NZBB8F1,
NZBC3F1,
NZBCF1,
NZBPWF1,
NZBSJLF1,
PW129SF1,
PWB6F1,
PWB8F1,
PWC3F1,
PWCF1,
PWNZBF1,
PWSJLF1,
SJL129SF1,
SJLB6F1,
SJLB8F1,
SJLC3F1,
SJLCF1,
SJLNZBF1,
SJLPWF1.
Vendor |
Mice
were
housed
in
JAX®
production
or
research
rooms
until
either
7-8
weeks
of
age
when
sample
collection
took
place.
The
F1
mice
are
acclimated
in
the
research
laboratory
mouse
room
and
aged
until
8-wk
of
age
before
testing.
BALB/cJ
inbred
strain
crossed
to
PWK/PhJ
wild
inbred
strain
is
the
only
breeding
that
did
not
yield
(CPWF1)
litters.
|
Ages
|
Mice
were
10-13
wks
of
age
on
the
day
of
sacrifice |
Feed
|
All
strains
maintained
on
a
sterilized
6%
fat
diet
(NIH316
formula,
Purina
item
#5K52)
from
age
of
weaning |
Water
|
Adjusted
with
hydrochloric
acid
to
pH
2.8-3.1
and
supplemented
with
menadione
sodium
bisulfite
(final
concentration
0.4
mg/ml)
to
compensate
for
potential
decrease
in
vitamin
K
activity
upon
autoclaving;
water
supplied
in
pinhole
type
bottles
(Thoren
Caging
Systems,
Inc.
Hazelton,
PA). |
Housing
|
Thoren
cages;
Thoren
Maxi
Miser
#3
cages
used
for
some
strains
as
either
part
of
a
PIV
(pressurized
individually
ventilated)
system
or
placed
on
conventional
racking. |
Room
temperature |
18
-
24°C |
Bedding |
Steam
autoclaved
white
pine
shavings |
Relative
humidity |
~50%. |
Photoperiod |
14
hrs
on
(generally
covering
the
hrs
of
4:30
AM
to
6:30
PM)
10
hrs
off |
Health
status
|
The
Jackson
Laboratory
Diagnostics
Laboratory
routinely
monitors
and
documents
animal
health
status
using
statistically
valid
sampling
procedures
and
standard
protocols.
(Sentinel
animals
are
tested).
Detailed
health
status
reports
for
each
animal
room
represented
from
this
project
are
on
file
and
available
on
request.
See
JAX
Mice
Quality
Control
Programs
or
the
JAX
Mice
Catalog
for
the
list
of
agents
monitored. |
