Ontology terms mapped to Xenakis2 measures:

  MA:0000009   adipose tissue
  MA:0000059   blood
  MA:0000358   liver
  MA:0002545   urine
  MP:0000188   abnormal circulating glucose level MGI
  MP:0000609   abnormal liver physiology MGI
  MP:0001259   abnormal body weight MGI
  MP:0001560   abnormal circulating insulin level MGI
  MP:0002136   abnormal kidney physiology MGI
  MP:0003959   abnormal lean body mass MGI
  MP:0005449   abnormal food intake MGI
  MP:0005452   abnormal adipose tissue amount MGI
  MP:0008872   abnormal physiological response to xenobiotic MGI
  MP:0009643   abnormal urine homeostasis MGI
  MP:0011923   abnormal bladder urine volume MGI
  MP:0011947   abnormal fluid intake MGI
  MP:0013275   abnormal xenobiotic metabolism MGI
  VT:0000188   blood glucose amount
  VT:0001259   body mass
  VT:0001422   drinking behavior trait
  VT:0001431   eating behavior trait
  VT:0001560   blood insulin amount
  VT:0001757   urine molecular composition trait
  VT:0002121   metabolism trait
  VT:0003620   urine output
  VT:0005452   adipose amount
  VT:0010482   body fat mass
  VT:0010483   body lean mass
  VT:0010487   response to xenobiotic stimulus trait
  VT:0010497   liver molecular composition trait
  VT:0010638   xenobiotic metabolism trait

Investigators	James G Xenakis       University of North Carolina,  Chapel Hill, NC Rebecca Fry       University of North Carolina,  Chapel Hill, NC Miroslav Stýblo       University of North Carolina,  Chapel Hill, NC Fernando Pardo-Manuel de Villena       University of North Carolina,  Chapel Hill, NC
Participants	Douillet C, Bell TA, Hock P, Farrington J, Liu T, Murphy CEY, Saraswatula A, Shaw GD, Nativio G, Shi Q, Venkatratnam A, Zou F
Contact	Fernando Pardo-Manuel de Villena     fernando@med.unc.edu     Lab web site
Funding Provided By	NIH ES029925, ES028721, ES031007, UNC Nutrition Obesity Research Center grant DK056350 (NIDDK), training grant T32ES007126.
Project type	Phenotype strain survey data set
MPD identifiers	Xenakis2     MPD:1125
Data changelog	No updates/corrections.       Initial release date: 02/2023.
Formatted citation	Click above to copy-paste the entire citation for this MPD web page.

The effects of chronic exposure to inorganic arsenic (iAs), a ubiquitous environmental contaminant, and its association with type 2 diabetes were investigated in a cohort of 75 DO mice. Male mice were exposed to iAs in drinking water (100 ppb) for 26 weeks, after which diabetic surrogate risk indicators such as fasting blood glucose and plasma insulin, and blood glucose and plasma insulin 15 minutes after glucose challenge were measured. Additionally, body composition was measured using magnetic resonance imaging and the concentrations of iAs and its methylated metabolites, in particular iAs speciation, were measured in liver and urine.

Experimental groups in this study:
• Inorganic arsenic (iAs)    

Procedures conducted:

• body weight		Baseline, then after iAs exposure for 26 wks
• intake monitoring		Food and water consumption, at baseline then at 1 to 4 wk intervals after iAs exposure
• MRI		Fat and lean tissue mass at baseline, and 12 and 24 wks after iAs exposure
• urinalysis		Urine volume at baseline, and 12 and 24 wks after iAs exposure
• metabolic panel		Blood glucose following 6 h fast, and after intraperitoneal glucose injection; at baseline and after iAs exposure
• hormone quantification		Blood insulin following 6 h fast, and after intraperitoneal glucose injection; at baseline and after iAs exposure
• metabolite quantification		iAs quantification in urine and liver, at 24 and 26 wks after iAs exposure. IAs speciation evaluated specifically monomethyl-arsenic acid (MMA) and dimethyl-arsenic acid (DMA)