Wittenburg3: Plasma lipids, cholesterol gallstones, and related gallstone phenotypes in an intercross between DBA/2J and PERA/EiJ (2005)

References   (chronological)

1.   Li R, Lyons MA, Wittenburg H, Paigen B, Churchill GA. Combining data from multiple inbred line crosses improves the power and resolution of quantitative trait loci mapping. Genetics. 2005 Mar;169(3):1699-709. Epub 2005 Jan 16.   PubMed 15654110     FullText

2.   Wittenburg H, Lyons MA, Li R, Kurtz U, Mössner J, Churchill GA, Carey MC, Paigen B. Association of a lithogenic Abcg5/Abcg8 allele on Chromosome 17 (Lith9) with cholesterol gallstone formation in PERA/EiJ mice. Mamm Genome. 2005 Jul;16(7):495-504.   PubMed 16151694  

3.   Wittenburg H, Lyons MA, Li R, Kurtz U, Wang X, Mössner J, Churchill GA, Carey MC, Paigen B. QTL mapping for genetic determinants of lipoprotein cholesterol levels in combined crosses of inbred mouse strains. J Lipid Res. 2006 Aug;47(8):1780-90. Epub 2006 May 9.   PubMed 16685081  


     
Wittenburg3 downloads
QTL projects have several available downloads.
Use the QTL Data Downloads button near the bottom of this page.
Participants H Wittenberg
Gary A Churchill       The Jackson Laboratory Bar Harbor, ME
With: Lyons MA, Li R, Kurtz U, Mössner J, Carey MC, Paigen B
ContactGary A Churchill     gary.churchill@jax.org
AffiliationChurchill Group QTL Archive
AcknowledgementsFunding provided by DK51568, DK36588, DK52911, DK34854, DK73687, GM70683, HL55001; and the American Physiological Society, American Liver Foundation, National Health and Medical Research Council of Australia, the Gastroenterological Society of Australia and Deutsche Forschungsgemeinschaft.
Project type QTL Archive data set
MPD identifiersWittenburg3     MPD:182
Data changelog No updates/corrections.       Initial release date: 01/2008.
An intercross between DBA/2J and PERA/EiJ was generated to identify QTLs for cholesterol gallstone susceptibility. At 6-8 wks of age, backcross progeny of both sexes were administered a high-fat (15%) diet for 8 wks and examined for gallstone formation and related phenotypes. Mice were fasted prior to sample collection. For detailed protocols see Wittenburg et al 2005.


QTL data set parameters:
Progenitor strains DBA/2J     PERA/EiJ    
Cross direction notation(PERAxDBA) F1 x (PERAxDBA) F1; (DBAxPERA) F1 x (DBAxPERA) F1
Cross typeF2 intercross
Population size 166 ♀     158 ♂
Age14-16wks


Measured phenotypes:       To access these data use the QTL Data Downloads button at bottom of page.
Select table page: First Prev Next Last
Data set MPD ID Procedure / Protocol Drug or Challenge Phenotype Measure Panel No. of Strains Sex Age Sample Size (Avg)
Wittenburg3 18201 body weight high fat diet  8wks body weight   [g]  bw QTL population both 14-16wks N=162
Wittenburg3 18202 organ weights high fat diet  8wks liver weight   [g]  liver_wt QTL population both 14-16wks N=162
Wittenburg3 18203 organ weights high fat diet  8wks percent of body weight that is liver   [%]  pct_liver_wt QTL population both 14-16wks N=162
Wittenburg3 18204 organ dimensions high fat diet  8wks gallbladder volume, log transformed   [log(µL)]  gallbladder_vol_log QTL population both 14-16wks N=162
Wittenburg3 18205 histopathology high fat diet  8wks gallstones, presence (score 0=absent, 1=present)   [score]  gallstone_presence QTL population both 14-16wks N=162
Wittenburg3 18206 histopathology high fat diet  8wks sandy gallstones, presence (score 0=absent, 1=present)   [score]  gallstone_sandy QTL population both 14-16wks N=162
Wittenburg3 18207 histopathology high fat diet  8wks solid gallstones, presence (score 0=absent, 1=present)   [score]  gallstone_solid QTL population both 14-16wks N=162
Wittenburg3 18208 histopathology high fat diet  8wks number of gallstones   [n]  gallstone_count QTL population both 14-16wks N=162
Wittenburg3 18209 histopathology high fat diet  8wks gallstone weight   [mg]  gallstone_wt QTL population both 14-16wks N=162
Wittenburg3 18210 histopathology high fat diet  8wks cholesterol monohydrate crystals (score 0=absent, 1-5=severity)   [score]  gallstone_crystals QTL population both 14-16wks N=162
Wittenburg3 18211 histopathology high fat diet  8wks gallbladder, mucin (score 0=absent, 1-5=severity)   [score]  gallbladder_mucin QTL population both 14-16wks N=162
Wittenburg3 18212 histopathology high fat diet  8wks gallstone score (score 0=absent, 1=crystals only, 2=sandy or solid gallstones)   [score]  gallstone_score QTL population both 14-16wks N=162
Wittenburg3 18213 histopathology high fat diet  8wks gallstone severity index (score 0-3, increasing severity)   [score]  gallstone_severity QTL population both 14-16wks N=162
Wittenburg3 18214 lipid profile high fat diet  8wks total cholesterol (plasma), log transformed   [log(mg/dL)]  chol_log QTL population both 14-16wks N=162
Wittenburg3 18215 lipid profile high fat diet  8wks HDL cholesterol (plasma)   [mg/dL]  HDL QTL population both 14-16wks N=162
Wittenburg3 18216 lipid profile high fat diet  8wks HDL cholesterol (plasma), log transformed   [log(mg/dL)]  HDL_log QTL population both 14-16wks N=162
Wittenburg3 18217 lipid profile high fat diet  8wks nonHDL cholesterol (plasma), log transformed   [log(mg/dL)]  nonHDL_log QTL population both 14-16wks N=162
Wittenburg3 18218 histopathology high fat diet  8wks gallstone weight if gallstones present   [mg]  gallstone_wt_if_present QTL population both 14-16wks N=162
Wittenburg3 18219 histopathology high fat diet  8wks gallstones presence score (0=absent, 1=present)  gallstone_present_absent QTL population both 14-16wks N=162
Wittenburg3 18220 histopathology high fat diet  8wks Revised subjective summary score (0-3)  score_new QTL population both 14-16wks N=162
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Associated genomic features
MGI-curated genes / genomic features that have been associated with this data set:
Hdlq10   Hdlq33   Hdlq34   Hdlq35   Hdlq36   Hdlq37   Hdlq38   Hdlq39   Lith12   Lith14   Lith15   Lith16   Lith17   Lith4   Lith9   Nhdlq10   Nhdlq8   Nhdlq9  


Project / data set:   Wittenburg3     a.k.a. Wittenburg_2005
Excel file:   Data_Description_PERAxDBA_Wittenburg2005.xlsx
CSV file:   Wittenburg2005_PERAxDBA_B37_Data.csv
Readme / text file:   readme_Wittenburg2005.txt