Lyons2: Gallstone susceptibility in F2 progeny of 129S1/SvImJ and CAST/EiJ (2003)

References   (chronological)

1.   Lyons MA, Wittenburg H, Li R, Walsh KA, Leonard MR, Churchill GA, Carey MC, Paigen B. New quantitative trait loci that contribute to cholesterol gallstone formation detected in an intercross of CAST/Ei and 129S1/SvImJ inbred mice. Physiol Genomics. 2003 Aug 15;14(3):225-39.   PubMed 12837957  

2.   Lyons MA, Wittenburg H, Li R, Walsh KA, Korstanje R, Churchill GA, Carey MC, Paigen B. Quantitative trait loci that determine lipoprotein cholesterol levels in an intercross of 129S1/SvImJ and CAST/Ei inbred mice. Physiol Genomics. 2004 Mar 12;17(1):60-8.   PubMed 14701919  

3.   Li R, Lyons MA, Wittenburg H, Paigen B, Churchill GA. Combining data from multiple inbred line crosses improves the power and resolution of quantitative trait loci mapping. Genetics. 2005 Mar;169(3):1699-709. Epub 2005 Jan 16.   PubMed 15654110     FullText


     
Lyons2 downloads
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Use the QTL Data Downloads button near the bottom of this page.
Participants M A Lyons
Gary A Churchill       The Jackson Laboratory Bar Harbor, ME
With: Wittenburg H, Li R, Walsh KA, Korstanje R, Carey MC, Paigen B
ContactGary A Churchill     gary.churchill@jax.org
AffiliationChurchill Group QTL Archive
AcknowledgementsThis work was supported in part by National Institute Health Grants DK-51568 (to B. Paigen), DK-36588, DK-52911, and DK-34854 (to M. C. Carey), and CA-34196 (core grant to the Jackson Laboratory). M. A. Lyons was supported by the American Physiological Society and the American Liver Foundation.
Project type QTL Archive data set
MPD identifiersLyons2     MPD:131
Data changelog No updates/corrections.       Initial release date: 01/2008.
To determine the genetic contribution to variation among cholesterol levels, an F2 intercross between RIIIS/J and 129S1/SvImJ strains was generated. 330 male mice from F2 population were fed an atherogenic (high-fat) diet for 8-12 wks. The total plasma, HDL and non-HDL were determined at the end of feeding period and some significant QTLs were identified in these phenotypes. For detailed protocols see Lyons et al 2004.


QTL data set parameters:
Progenitor strains 129S1/SvImJ     CAST/EiJ    
Cross direction notation(CASTx129) F1 x (CASTx129) F1; (129xCAST) F1 x (129xCAST) F1
Cross typeF2 intercross
Population size 0 ♀     277 ♂
Age16-18wks


Measured phenotypes:       To access these data use the QTL Data Downloads button at bottom of page.
Select table page: First Prev Next Last
Data set MPD ID Procedure / Protocol Drug or Challenge Phenotype Measure Panel No. of Strains Sex Age Sample Size (Avg)
Lyons2 13101 organ weights high fat diet  10wks liver weight   [g]  liver_wt QTL population m 16-18wks N=277
Lyons2 13102 organ weights high fat diet  10wks liver-to-bw ratio   [ratio]  liver_bw_ratio QTL population m 16-18wks N=277
Lyons2 13103 organ dimensions high fat diet  10wks gallbladder, volume   [µL]  gallbladder_vol QTL population m 16-18wks N=277
Lyons2 13104 histopathology high fat diet  10wks gallbladder, mucin (score 0-4)   [score]  gallbladder_mucin QTL population m 16-18wks N=277
Lyons2 13105 histopathology high fat diet  10wks gallbladder bile characteristics (score 0=no crystals, 1=crystals present, 2=solid or sandy gallstones present)   [score]  bile_score QTL population m 16-18wks N=277
Lyons2 13106 histopathology high fat diet  10wks cholesterol monohydrate crystals (score 0=absent, 1-4=increasing severity)   [score]  gallstone_crystals QTL population m 16-18wks N=277
Lyons2 13107 histopathology high fat diet  10wks sandy gallstones (score 0=absence, 1-4=severity)   [score]  gallstone_sandy QTL population m 16-18wks N=277
Lyons2 13108 histopathology high fat diet  10wks solid gallstones (score 0=absence, 1-5=severity)   [score]  gallstone_solid QTL population m 16-18wks N=277
Lyons2 13109 histopathology high fat diet  10wks number of solid gallstones   [n]  gallstone_count QTL population m 16-18wks N=277
Lyons2 13110 histopathology high fat diet  10wks weight of solid gallstones   [mg]  gallstone_weight QTL population m 16-18wks N=277
Lyons2 13111 lipid profile high fat diet  10wks HDL cholesterol (plasma)   [mg/dL]  HDL QTL population m 16-18wks N=277
Lyons2 13112 lipid profile high fat diet  10wks non-HDL cholesterol (plasma)   [mg/dL]  nonHDL QTL population m 16-18wks N=277
Lyons2 13113 lipid profile high fat diet  10wks total cholesterol (plasma), log transformed   [log(mg/dL)]  chol QTL population m 16-18wks N=277
Lyons2 13114 histopathology high fat diet  10wks solid gallstones, presence (score 0=absent, 1=present)   [score]  Solid QTL population m 16-18wks N=277
To see more rows use the paging control at top.

Associated genomic features
MGI-curated genes / genomic features that have been associated with this data set:
Chol7   Chol8   Chol9   Gbvq1   Hdlq10   Hdlq5   Lch   Lch2   Lith12   Lith13   Lith14   Lith6   Nhdlq1   Nhdlq2  


Project / data set:   Lyons2     a.k.a. Lyons_2003b
Excel file:   Data_Description_CASTx129_Lyons2003b.xlsx
CSV file:   Lyons2003b_CASTx129_B37_Data.csv
Readme / text file:   readme_Lyons2003b.txt
QC plots, figures, etc.:   QCReport_Lyons2003b.pdf